Sitotoksisitas bahan restorasi cyanoacrylate pada variasi perbandingan powder dan liquid menggunakan MTT assay (Cytotoxicity of the cyanoacrylate restoration material with variation of powder and liquid ratio by using MTT assay)

• Main Author: Asti Meizarini (Author)
• Format: Book
• Published: Universitas Airlangga, 2006-03-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 1978-3728; 2442-9740; 10.20473/j.djmkg.v38.i1.p20-24
• Keywords: cytotoxicity; cyanoacrylate restoration material; powder and liquid ratio; MTT assay; Dentistry; RK1-715
• Source: Dental Journal, Vol 38, Iss 1, Pp 20-24 (2006)

<span style="font-family: TribuneItalic; font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><em>The requirements for dental material include not toxic, not irritant, no carcinogenic potential, nor cause an allergic response </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><em>with the use in oral cavity. The cyanoacrylate restoration material has certain substance that can be toxic. Because of the ratio </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><em>amount of powder and liquid is not known, it can lead the restoration more toxic. The purpose of this study was to know the </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><em>cytotoxicity of the cyanoacrylate restoration material with different variation of powder and liquid ratio using MTT assay. Six </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><em>cylinder samples of 5 mm in diameter and 2 mm in thickness were used for each group of 1:1.00; 1:0.75; 1:0.50 powder and liquid </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><em>ratio of cyanoacrylate restoration materials. Each of samples was immersed in eppendorf micro tubes consisting of media culture. </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><em>After 24 hour, the immersion of media culture was used to investigate the cytotoxic effect to BHK-21 cell lines by MTT assay method. </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><em>The density of optic formazan indicated the amount of living cells. All data were statistically analyzed by one-way ANOVA and HSD. </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><em>The results showed that the percentage of living cells amount of powder and liquid ratio 1:1.00; 1:0.75; 1:0.50 were 98.59%; </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><em>95.76%; 94.92% respectively. There was a significant difference between 1:1.00 and 1:0.50 group ratio. The conclusion was that </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><em>the cytotoxicity between 1:1.00 and 1:0.50 powder and liquid ratio of cyanoacrylate restoration materials in this study decreased.</em></span></span></span></span></span></span></span></span></span></span><br style="font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px;" /></span>