Reporting detection of Chlamydia trachomatis DNA in tissues of neonatal death cases

• Main Authors: Maria Hernandez Trejo (Author), Norma E. Herrera Gonzalez (Author), Marcos R. Escobedo Guerra (Author), M. de Jesus de Haro Cruz (Author), Elsa R. Moreno-Verduzco (Author), Marcela Lopez-Hurtado (Author), Fernando M. Guerra-Infante (Author)
• Format: Book
• Published: Brazilian Society of Pediatrics, 2014-03-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

Objective: to determine whether C. trachomatis was present in neonates with infection, but without an isolated pathogen, who died during the first week of life. Methods: early neonatal death cases whose causes of death had been previously adjudicated by the institutional mortality committee were randomly selected. Endpoint and real-time poly-merase chain reaction of the C. trachomatis omp1 gene was used to blindly identify the presenceof chlamydial DNA in the paraffinized samples of five organs (from authorized autopsies) ofeach of the dead neonates. Additionally, differential diagnoses were conducted by amplifyinga fragment of the 16S rRNA of Mycoplasma spp. Results: in five cases (35.7%), C. trachomatis DNA was found in one or more organs. Severe neonatal infection was present in three cases; one of them corresponded to genotype D of C. trachomatis. Interestingly, another case fulfilled the same criteria but had a positive polymerase chain reaction for Mycoplasma hominis, a pathogen known to produce sepsis in newborns. Conclusion: the use of molecular biology techniques in these cases of early infant mortality demonstrated that C. trachomatis could play a role in the development of severe infection and in early neonatal death, similarly to that observed with Mycoplasma hominis. Further study is required to determine the pathogenesis of this perinatal infection.

Item Description

2255-5536
10.1016/j.jpedp.2013.08.007