Utilizing Proteomic Approach to Analyze Potential Antioxidant Proteins in Plant against Irradiation

• Main Authors: Ming-Hui Yang (Author), Yi-Shan Lu (Author), Tzu-Chuan Ho (Author), Daniel Hueng-Yuan Shen (Author), Ying-Fong Huang (Author), Kuo-Pin Chuang (Author), Cheng-Hui Yuan (Author), Yu-Chang Tyan (Author)
• Format: Book
• Published: MDPI AG, 2022-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

Gamma-ray irradiation is an effective and clean method of sterilization by inactivating microorganisms. It can also be applied to induce anti-oxidants for future application. In this study, the mung bean (<i>Vigna radiata</i>) was exposed to gamma-ray irradiation under the dose of 0, 5 or 10 kGy. With increasing irradiation doses, the concentrations of malondiadehyde decreased while the levels of total flavonoids and DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity increased. It has been shown that consuming flavonoids can provide protective effects. In addition, proteomic analysis identified several proteins having anti-oxidant activities in the 5 kGy irradiated group. These proteins are Apocytochrome <i>f</i>, Systemin receptor SR 160, DELLA protein DWARF8, DEAD-box ATP-dependent RNA helicase 9, ζ-carotene desaturase (ZDS), and Floral homeotic protein AGAMOUS. Our findings indicate that plants contain a variety of phytochemicals and antioxidant proteins which may effectively prevent oxidative stress caused by irradiated peroxidation.

Item Description

10.3390/antiox11122498
2076-3921