New Lignanamides with Antioxidant and Anti-Inflammatory Activities Screened Out and Identified from <i>Warburgia ugandensis</i> Combining Affinity Ultrafiltration LC-MS with SOD and XOD Enzymes
<i>Warburgia ugandensis</i>, also known as "green heart," is widely used for the treatment of various diseases as a traditional ethnomedicinal plant in local communities in Africa. In this work, 9 and 12 potential superoxide dismutase (SOD) and xanthine oxidase (XOD) ligands fr...
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| Main Authors: | , , , , |
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| Format: | Book |
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MDPI AG,
2021-03-01T00:00:00Z.
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| Summary: | <i>Warburgia ugandensis</i>, also known as "green heart," is widely used for the treatment of various diseases as a traditional ethnomedicinal plant in local communities in Africa. In this work, 9 and 12 potential superoxide dismutase (SOD) and xanthine oxidase (XOD) ligands from <i>W. ugandensis</i> were quickly screened out by combining SOD and XOD affinity ultrafiltration with LC-MS, respectively. In this way, four new lignanamides (compounds <b>11</b>-<b>14</b>) and one new macrocyclic glycoside (compound <b>5</b>), along with three known compounds (compounds <b>1</b>, <b>3</b>, and <b>7</b>), were isolated and identified firstly in this species. The structures of the new compounds were elucidated by spectroscopic analysis, including NMR and UPLC-QTOF-MS/MS. Among these compounds, compound <b>14</b> showed the highest 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azinobis-(3-ethylbenzthiazoline)-6-sulfonic acid (ABTS) radical scavenging activities, and total ferric-reducing antioxidant power (FRAP) with IC<sub>50</sub> values of 6.405 ± 0.362 µM, 5.381 ± 0.092 µM, and 17.488 ± 1.625 mmol TE/g, respectively. Moreover, compound <b>14</b> displayed the highest inhibitory activity on cyclooxygenase-2 (COX-2) with IC<sub>50</sub> value of 0.123 ± 0.004 µM, and the ranking order of other compounds' IC<sub>50</sub> values was <b>13</b> > <b>11</b> > <b>7</b> > <b>1</b> > <b>12</b>. The present study suggested that lignanamides might represent interesting new characteristic functional components of <i>W. ugandensis</i> to exert remarkable antioxidant and anti-inflammatory activities. Moreover, compound <b>14</b>, a new arylnaphthalene lignanamide, would be a highly potential natural antioxidant and anti-inflammatory agent from <i>W. ugandensis</i>. |
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| Item Description: | 10.3390/antiox10030370 2076-3921 |