New Lignanamides with Antioxidant and Anti-Inflammatory Activities Screened Out and Identified from <i>Warburgia ugandensis</i> Combining Affinity Ultrafiltration LC-MS with SOD and XOD Enzymes

• Main Authors: Xiao-Cui Zhuang (Author), Gui-Lin Chen (Author), Ye Liu (Author), Yong-Li Zhang (Author), Ming-Quan Guo (Author)
• Format: Book
• Published: MDPI AG, 2021-03-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 10.3390/antiox10030370; 2076-3921
• Keywords: <i>Warburgia ugandensis</i>; superoxide dismutase; xanthine oxidase; cyclooxygenase-2; ultrafiltration; antioxidant; Therapeutics. Pharmacology; RM1-950
• Source: Antioxidants, Vol 10, Iss 3, p 370 (2021)

<i>Warburgia ugandensis</i>, also known as "green heart," is widely used for the treatment of various diseases as a traditional ethnomedicinal plant in local communities in Africa. In this work, 9 and 12 potential superoxide dismutase (SOD) and xanthine oxidase (XOD) ligands from <i>W. ugandensis</i> were quickly screened out by combining SOD and XOD affinity ultrafiltration with LC-MS, respectively. In this way, four new lignanamides (compounds <b>11</b>-<b>14</b>) and one new macrocyclic glycoside (compound <b>5</b>), along with three known compounds (compounds <b>1</b>, <b>3</b>, and <b>7</b>), were isolated and identified firstly in this species. The structures of the new compounds were elucidated by spectroscopic analysis, including NMR and UPLC-QTOF-MS/MS. Among these compounds, compound <b>14</b> showed the highest 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azinobis-(3-ethylbenzthiazoline)-6-sulfonic acid (ABTS) radical scavenging activities, and total ferric-reducing antioxidant power (FRAP) with IC₅₀ values of 6.405 ± 0.362 µM, 5.381 ± 0.092 µM, and 17.488 ± 1.625 mmol TE/g, respectively. Moreover, compound <b>14</b> displayed the highest inhibitory activity on cyclooxygenase-2 (COX-2) with IC₅₀ value of 0.123 ± 0.004 µM, and the ranking order of other compounds' IC₅₀ values was <b>13</b> > <b>11</b> > <b>7</b> > <b>1</b> > <b>12</b>. The present study suggested that lignanamides might represent interesting new characteristic functional components of <i>W. ugandensis</i> to exert remarkable antioxidant and anti-inflammatory activities. Moreover, compound <b>14</b>, a new arylnaphthalene lignanamide, would be a highly potential natural antioxidant and anti-inflammatory agent from <i>W. ugandensis</i>.