Screening Differential Expression Profiles of Urinary microRNAs in a Gentamycin-Induced Acute Kidney Injury Canine Model
microRNAs (miRNAs) are promising biomarkers for different pathological models because of their stable and detectable characters in biofluids. Here, we collected urine samples from 5 beagle dogs on the 3th, 6th, and 12th day in an acute kidney injury (AKI) caused by gentamycin. miRNA levels were meas...
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| Format: | Book |
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MDPI AG,
2023-04-01T00:00:00Z.
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|---|---|---|---|
| 001 | doaj_8c3af6ae2e3948e7a6c7f17194bc5cb7 | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a Bo Sun |e author |
| 700 | 1 | 0 | |a Liang Chen |e author |
| 700 | 1 | 0 | |a Zhe Qu |e author |
| 700 | 1 | 0 | |a Yan-Wei Yang |e author |
| 700 | 1 | 0 | |a Yu-Fa Miao |e author |
| 700 | 1 | 0 | |a Rui-Li Wang |e author |
| 700 | 1 | 0 | |a Xiao-Bing Zhou |e author |
| 700 | 1 | 0 | |a Bo Li |e author |
| 245 | 0 | 0 | |a Screening Differential Expression Profiles of Urinary microRNAs in a Gentamycin-Induced Acute Kidney Injury Canine Model |
| 260 | |b MDPI AG, |c 2023-04-01T00:00:00Z. | ||
| 500 | |a 10.3390/kidneydial3020019 | ||
| 500 | |a 2673-8236 | ||
| 520 | |a microRNAs (miRNAs) are promising biomarkers for different pathological models because of their stable and detectable characters in biofluids. Here, we collected urine samples from 5 beagle dogs on the 3th, 6th, and 12th day in an acute kidney injury (AKI) caused by gentamycin. miRNA levels were measured with high-throughput sequencing and the results were then differentially investigated. Gene Ontology (GO) and KEGG pathway analysis were performed to analyze potential target genes corresponding to the differentially expressed miRNAs (DE-miRNAs). Relationships between hub genes and DE-miRNAs were analyzed with STRING and Cytoscape. We identified 234 DE-miRNAs 3, 6, and 12 days after gentamycin treatment (<i>p</i> < 0.05). Top 10 up- and down-regulated candidate target genes of DE-miRNAs were predicted by overlapping TargetScan and miRanda results). GO and KEGG analyses for DE-miRNAs demonstrated that the DE-miRNAs target genes are mainly involved in kidney injury-related pathways, such as the insulin signaling pathway, oxytocin signaling pathway, and hedgehog signaling pathway. The network of miRNA-hub genes suggests that miR-452, miR-106a, and 106b participate in regulating the largest number of hub genes. We evaluated the miRNA signature via a canine model built by gentamycin-caused acute kidney injury. Our results represent a valuable resource for evaluating miRNAs as biomarkers of renal toxicity. | ||
| 546 | |a EN | ||
| 690 | |a acute kidney injury | ||
| 690 | |a high-throughput sequencing | ||
| 690 | |a microRNA signature | ||
| 690 | |a biomarker | ||
| 690 | |a Internal medicine | ||
| 690 | |a RC31-1245 | ||
| 655 | 7 | |a article |2 local | |
| 786 | 0 | |n Kidney and Dialysis, Vol 3, Iss 2, Pp 204-218 (2023) | |
| 787 | 0 | |n https://www.mdpi.com/2673-8236/3/2/19 | |
| 787 | 0 | |n https://doaj.org/toc/2673-8236 | |
| 856 | 4 | 1 | |u https://doaj.org/article/8c3af6ae2e3948e7a6c7f17194bc5cb7 |z Connect to this object online. |