Isolation and identification of three new chromones from the leaves of Pimenta dioica with cytotoxic, oestrogenic and anti-oestrogenic effects
Context: Pimenta dioica (L.) Merr. (Myrtaceae) is used in Costa Rican traditional medicine for women's health. Our previous work showed that P. dioica extracts were oestrogenic. Objectives: This work identifies phytochemicals from P. dioica that are responsible for the plant's oestrogen-li...
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Taylor & Francis Group,
2018-01-01T00:00:00Z.
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LEADER | 00000 am a22000003u 4500 | ||
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001 | doaj_8c8f9f5aeaef42f3bd70016fcca807b4 | ||
042 | |a dc | ||
100 | 1 | 0 | |a Brian J. Doyle |e author |
700 | 1 | 0 | |a Temitope O. Lawal |e author |
700 | 1 | 0 | |a Tracie D. Locklear |e author |
700 | 1 | 0 | |a Lorraina Hernandez |e author |
700 | 1 | 0 | |a Alice L. Perez |e author |
700 | 1 | 0 | |a Udeshi Patel |e author |
700 | 1 | 0 | |a Shitalben Patel |e author |
700 | 1 | 0 | |a Gail B. Mahady |e author |
245 | 0 | 0 | |a Isolation and identification of three new chromones from the leaves of Pimenta dioica with cytotoxic, oestrogenic and anti-oestrogenic effects |
260 | |b Taylor & Francis Group, |c 2018-01-01T00:00:00Z. | ||
500 | |a 1388-0209 | ||
500 | |a 1744-5116 | ||
500 | |a 10.1080/13880209.2018.1448873 | ||
520 | |a Context: Pimenta dioica (L.) Merr. (Myrtaceae) is used in Costa Rican traditional medicine for women's health. Our previous work showed that P. dioica extracts were oestrogenic. Objectives: This work identifies phytochemicals from P. dioica that are responsible for the plant's oestrogen-like activities. Materials and methods: P. dioica leaves were collected in Costa Rica in 2005. Fractions resulting from chromatographic separation of a methanol extract were tested at 50 μg/mL in a competitive oestrogen receptor-binding assay. Active compounds were isolated by HPLC and identified by NMR and MS. Pure compounds were tested at 1 μM in the oestrogen-responsive SEAP reporter gene assay. The effects on cell viability, cytotoxicity and apoptosis were investigated in breast cancer (MCF-7 and SK-BR3) and gastric cancer (AGS and NCI-N87) cell lines using the ApoTox-Glo and Caspase-Glo assays and qPCR. Results: Quercitrin and three new chromones, including a 2-phenoxychromone, 6,8-di-C-methylcapillarisin (1) were isolated and identified. Compound 1 caused a 6.2-fold increase in SEAP expression at 1 μM (p < 0.05). This activity was blocked by the ER antagonist ICI 182,780. Compound 2 caused a 6.0-fold increase in SEAP, inhibited the growth of MCF-7, AGS and NCI-N87 cells (IC50 54.27, 38.13 and 51.22 μg/mL, respectively), and induced apoptosis via caspase 8 and increased the Bax/Bcl-2 mRNA ratio in MCF-7 cells. Compound 3 was anti-oestrogenic in MCF-7 cells. Discussion and conclusions: Compounds from P. dioica have oestrogenic, anti-oestrogenic and cytotoxic effects that may explain the ethnomedical use of this plant. | ||
546 | |a EN | ||
690 | |a allspice | ||
690 | |a apoptosis | ||
690 | |a breast cancer | ||
690 | |a caspase | ||
690 | |a costa rica | ||
690 | |a ethnomedicine | ||
690 | |a oestrogen | ||
690 | |a latin america | ||
690 | |a menopause | ||
690 | |a Therapeutics. Pharmacology | ||
690 | |a RM1-950 | ||
655 | 7 | |a article |2 local | |
786 | 0 | |n Pharmaceutical Biology, Vol 56, Iss 1, Pp 235-244 (2018) | |
787 | 0 | |n http://dx.doi.org/10.1080/13880209.2018.1448873 | |
787 | 0 | |n https://doaj.org/toc/1388-0209 | |
787 | 0 | |n https://doaj.org/toc/1744-5116 | |
856 | 4 | 1 | |u https://doaj.org/article/8c8f9f5aeaef42f3bd70016fcca807b4 |z Connect to this object online. |