Isolation and identification of three new chromones from the leaves of Pimenta dioica with cytotoxic, oestrogenic and anti-oestrogenic effects

Context: Pimenta dioica (L.) Merr. (Myrtaceae) is used in Costa Rican traditional medicine for women's health. Our previous work showed that P. dioica extracts were oestrogenic. Objectives: This work identifies phytochemicals from P. dioica that are responsible for the plant's oestrogen-li...

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Main Authors: Brian J. Doyle (Author), Temitope O. Lawal (Author), Tracie D. Locklear (Author), Lorraina Hernandez (Author), Alice L. Perez (Author), Udeshi Patel (Author), Shitalben Patel (Author), Gail B. Mahady (Author)
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Published: Taylor & Francis Group, 2018-01-01T00:00:00Z.
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100 1 0 |a Brian J. Doyle  |e author 
700 1 0 |a Temitope O. Lawal  |e author 
700 1 0 |a Tracie D. Locklear  |e author 
700 1 0 |a Lorraina Hernandez  |e author 
700 1 0 |a Alice L. Perez  |e author 
700 1 0 |a Udeshi Patel  |e author 
700 1 0 |a Shitalben Patel  |e author 
700 1 0 |a Gail B. Mahady  |e author 
245 0 0 |a Isolation and identification of three new chromones from the leaves of Pimenta dioica with cytotoxic, oestrogenic and anti-oestrogenic effects 
260 |b Taylor & Francis Group,   |c 2018-01-01T00:00:00Z. 
500 |a 1388-0209 
500 |a 1744-5116 
500 |a 10.1080/13880209.2018.1448873 
520 |a Context: Pimenta dioica (L.) Merr. (Myrtaceae) is used in Costa Rican traditional medicine for women's health. Our previous work showed that P. dioica extracts were oestrogenic. Objectives: This work identifies phytochemicals from P. dioica that are responsible for the plant's oestrogen-like activities. Materials and methods: P. dioica leaves were collected in Costa Rica in 2005. Fractions resulting from chromatographic separation of a methanol extract were tested at 50 μg/mL in a competitive oestrogen receptor-binding assay. Active compounds were isolated by HPLC and identified by NMR and MS. Pure compounds were tested at 1 μM in the oestrogen-responsive SEAP reporter gene assay. The effects on cell viability, cytotoxicity and apoptosis were investigated in breast cancer (MCF-7 and SK-BR3) and gastric cancer (AGS and NCI-N87) cell lines using the ApoTox-Glo and Caspase-Glo assays and qPCR. Results: Quercitrin and three new chromones, including a 2-phenoxychromone, 6,8-di-C-methylcapillarisin (1) were isolated and identified. Compound 1 caused a 6.2-fold increase in SEAP expression at 1 μM (p < 0.05). This activity was blocked by the ER antagonist ICI 182,780. Compound 2 caused a 6.0-fold increase in SEAP, inhibited the growth of MCF-7, AGS and NCI-N87 cells (IC50 54.27, 38.13 and 51.22 μg/mL, respectively), and induced apoptosis via caspase 8 and increased the Bax/Bcl-2 mRNA ratio in MCF-7 cells. Compound 3 was anti-oestrogenic in MCF-7 cells. Discussion and conclusions: Compounds from P. dioica have oestrogenic, anti-oestrogenic and cytotoxic effects that may explain the ethnomedical use of this plant. 
546 |a EN 
690 |a allspice 
690 |a apoptosis 
690 |a breast cancer 
690 |a caspase 
690 |a costa rica 
690 |a ethnomedicine 
690 |a oestrogen 
690 |a latin america 
690 |a menopause 
690 |a Therapeutics. Pharmacology 
690 |a RM1-950 
655 7 |a article  |2 local 
786 0 |n Pharmaceutical Biology, Vol 56, Iss 1, Pp 235-244 (2018) 
787 0 |n http://dx.doi.org/10.1080/13880209.2018.1448873 
787 0 |n https://doaj.org/toc/1388-0209 
787 0 |n https://doaj.org/toc/1744-5116 
856 4 1 |u https://doaj.org/article/8c8f9f5aeaef42f3bd70016fcca807b4  |z Connect to this object online.