Evaluasi karakteristik abu sekam padi dengan kitosan molekul tinggi nanopartikel sebagai bahan dentinogenesis (Characteristic evaluation of rice husk ash with chitosan high molecule nanoparticle as dentinogenesis material)

• Main Authors: Pretty Farida Sinta Silalahi (Author), Trimurni Abidin (Author), Harry Agusnar (Author)
• Format: Book
• Published: Universitas Airlangga, 2014-06-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 1978-3728; 2442-9740; 10.20473/j.djmkg.v47.i2.p63-66
• Keywords: Rice husk ash nanoparticles (RHAn); high molecular chitosan nanoparticles (HMCn); scanning electron microscopy (SEM); Abu sekam padi nanopartikel (ASPn); kitosan molekul tinggi nanopartikel (KMTn); scanning electron microscopy (SEM); Dentistry; RK1-715
• Source: Dental Journal, Vol 47, Iss 2, Pp 63-66 (2014)

<p><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Background: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Mineral trioxide aggregate (MTA) and resin modified glass ionomer cement (RMGIC) are the material used for </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>indirect and direct pulp capping due to biocompatibility, but these materials have many shortcomings. Mineral trioxide aggregate </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>contains a little amount of arsenic and has long setting time, while HEMA containing RMGIC are cytotoxic. Rice husk ash nanoparticles </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>(RHAn) is a potential source of silica. High molecular chitosan nanoparticles (HMCn) can stimulate the formation of reparative dentin. </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Combination of these two materials is biocompatible and have good sealing ability. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Purpose: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>This study was aimed to study RHAn </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>+ HMCn used as biomaterials for prevention of pulpodentinal complex by examined at the microstructure of dentin surfaces applied </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>with RHAn + HMCn. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Methods: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Twenty-four mandibular premolar teeth extracted for orthodontic purposes, were made cavity class </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>I preparation with 3 mm depth above the cemento-enamel junction (CEJ). Then each tooth was cut in bucco-lingual direction and </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>each part was cut using a cervical disc bur. Samples were divided into 3 groups, group I the teeth were applied with MTA; group II </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>the teeth were applied RMGIC; group III the teeth were apllied with RHAn + HMCn. Characterization was done by using Scanning </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Electron Microscopy (SEM) on the interface between test material and dentin adjacent to the pulp to see surface microstructure. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Results: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Material microstructure of RHAn + HMCn applied to the dentine showed tags like structure which was more significant than </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>MTA. RHAn + HMCn showed to have better sealing ability than MTA. Porosity of ASPn + HMCn was less than MTA and RMGIC. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Conclusion: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>The study suggested that the combined RHAn + HMCn biomaterials could be used as an active biomaterial that can </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>maintain the integrity of pulp dentinal complex.</em></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></p><p><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Latar belakang: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Mineral trioksida agregat (MTA) dan semen ionomer kaca modifikasi resin (SIKMR) adalah bahan yang </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>digunakan untuk pulp capping langsung dan tidak langsung karena biokompatibel, namun bahan ini memiliki banyak kekurangan. </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Trioksida Mineral agregat mengandung sejumlah kecil arsenik dan setting time-nya lama, sementara HEMA dalam SIKMR bersifat </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>sitotoksik. Abu sekam padi nanopartikel (ASPn) merupakan sumber potensial dari silika. Kitosan molekul tinggi nanopartikel (KMTn) </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>dapat merangsang pembentukan dentin reparatif. Kombinasi dari kedua bahan tersebut memiliki sifat biokompatibel dan memiliki </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>kemampuan pelapisan yang baik. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Tujuan: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Penelitian ini bertujuan untuk menganalisa karakteristik mikrostruktur hubungan permukaan </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>abu sekam padi dengan kitosan molekul tinggi nanopartikel pada jaringan dentin untuk melindungi jaringan pulpodential kompleks. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Metode: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Dua puluh empat gigi premolar mandibula yang diekstraksi untuk tujuan ortodontik digunakan sebagai sampel, gigi dibuat </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>preparasi kavitas klas I dengan kedalaman 3 mm di atas cemento enamel junction (CEJ). Kemudian masing-masing gigi dibelah dua</em></span></span></span></span></span></span></span></span></span></span></span></span></span> <span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>arah bucco-lingual dan setiap bagian dipotong menggunakan disc bur servikal. Sampel dibagi 3 kelompok, kelompok I diaplikasikan </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>MTA, kelompok II diaplikasikan SIKMR, kelompok III diaplikasikan ASPn + KMTn.

Karakterisasi dilakukan dengan menggunakan </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Scanning Electron Microscopy (SEM) pada interface antara bahan uji dan dentin berdekatan dengan pulp untuk melihat struktur </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>mikro permukaan. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Hasil: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Microstructure bahan ASPn + KMTn yang diaplikasi pada dentin menunjukkan struktur seperti tag yang </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>lebih signifikan daripada MTA. ASPn + KMTn menunjukkan kemampuan pelapisan yang lebih baik dari MTA. Porositas ASPn + </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>KMTn lebih sedikit dari MTA dan SIKMR. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Simpulan: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Penelitian ini menunjukkan bahwa kombinasi biomaterial ASPn + KMTn dapat </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>digunakan sebagai biomaterial aktif yang dapat menjaga integritas pulpa.</em></span></span></span></span></span></span></span></span></span></span></span></p>