A corrosive oesophageal burn model in rats: Double-lumen central venous catheter usage

• Main Authors: Vedat Bakan (Author), Harun Çiralik (Author), Seyfi Kartal (Author)
• Format: Book
• Published: Wolters Kluwer Medknow Publications, 2015-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 0189-6725; 0974-5998; 10.4103/0189-6725.172560
• Keywords: Corrosive burn; oesophagus; method; Pediatrics; RJ1-570; Surgery; RD1-811
• Source: African Journal of Paediatric Surgery, Vol 12, Iss 4, Pp 247-250 (2015)

Background: We aimed to create a new and less invasive experimental corrosive oesophageal burn model using a catheter without a gastric puncture (gastrotomy). Materials and Methods: We conducted the study with two groups composed of 8 male rats. The experimental oesophageal burn was established by the application of 10% sodium hydroxide to the distal oesophagus under a pressure of 20 cmH 2 O, via 5-F double-lumen central venous catheter without a gastrotomy. The control group was given 0.9% sodium chloride. All rats were killed 24 h after administration of NaOH or 0.9% NaCl. Histologic damage to oesophageal tissue was scored by a single pathologist blind to groups. Results: The rats in the control group were observed to have no pathological changes. Corrosive oesophagitis (tissue congestion, oedema, inflammation, ulcer and necrosis) was observed in rats exposed to NaOH. Conclusion: We believe that an experimental corrosive oesophageal burn can safely be created under same hydrostatic pressure without a gastric puncture using this model.