A recombinant Mycobacterium smegmatis-based surface display system for developing the T cell-based COVID-19 vaccine
The immune escape mutations of SARS-CoV-2 variants emerged frequently, posing a new challenge to weaken the protective efficacy of current vaccines. Thus, the development of novel SARS-CoV-2 vaccines is of great significance for future epidemic prevention and control. We herein reported constructing...
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Taylor & Francis Group,
2023-01-01T00:00:00Z.
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| 001 | doaj_8d5fc53a2a0b4eed9df1510b3e7f37d6 | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a Ziyu Wen |e author |
| 700 | 1 | 0 | |a Cuiting Fang |e author |
| 700 | 1 | 0 | |a Xinglai Liu |e author |
| 700 | 1 | 0 | |a Yan Liu |e author |
| 700 | 1 | 0 | |a Minchao Li |e author |
| 700 | 1 | 0 | |a Yue Yuan |e author |
| 700 | 1 | 0 | |a Zirong Han |e author |
| 700 | 1 | 0 | |a Congcong Wang |e author |
| 700 | 1 | 0 | |a Tianyu Zhang |e author |
| 700 | 1 | 0 | |a Caijun Sun |e author |
| 245 | 0 | 0 | |a A recombinant Mycobacterium smegmatis-based surface display system for developing the T cell-based COVID-19 vaccine |
| 260 | |b Taylor & Francis Group, |c 2023-01-01T00:00:00Z. | ||
| 500 | |a 2164-5515 | ||
| 500 | |a 2164-554X | ||
| 500 | |a 10.1080/21645515.2023.2171233 | ||
| 520 | |a The immune escape mutations of SARS-CoV-2 variants emerged frequently, posing a new challenge to weaken the protective efficacy of current vaccines. Thus, the development of novel SARS-CoV-2 vaccines is of great significance for future epidemic prevention and control. We herein reported constructing the attenuated Mycobacterium smegmatis (M. smegmatis) as a bacterial surface display system to carry the spike (S) and nucleocapsid (N) of SARS-CoV-2. To mimic the native localization on the surface of viral particles, the S or N antigen was fused with truncated PE_PGRS33 protein, which is a transportation component onto the cell wall of Mycobacterium tuberculosis (M.tb). The sub-cellular fraction analysis demonstrated that S or N protein was exactly expressed onto the surface (cell wall) of the recombinant M. smegmatis. After the immunization of the M. smegmatis-based COVID-19 vaccine candidate in mice, S or N antigen-specific T cell immune responses were effectively elicited, and the subsets of central memory CD4+ T cells and CD8+ T cells were significantly induced. Further analysis showed that there were some potential cross-reactive CTL epitopes between SARS-CoV-2 and M.smegmatis. Overall, our data provided insights that M. smegmatis-based bacterial surface display system could be a suitable vector for developing T cell-based vaccines against SARS-CoV-2 and other infectious diseases. | ||
| 546 | |a EN | ||
| 690 | |a mycobacterium smegmatis | ||
| 690 | |a bacterial surface display system | ||
| 690 | |a sars-cov-2 | ||
| 690 | |a vaccine | ||
| 690 | |a t cell immunity | ||
| 690 | |a Immunologic diseases. Allergy | ||
| 690 | |a RC581-607 | ||
| 690 | |a Therapeutics. Pharmacology | ||
| 690 | |a RM1-950 | ||
| 655 | 7 | |a article |2 local | |
| 786 | 0 | |n Human Vaccines & Immunotherapeutics, Vol 19, Iss 1 (2023) | |
| 787 | 0 | |n http://dx.doi.org/10.1080/21645515.2023.2171233 | |
| 787 | 0 | |n https://doaj.org/toc/2164-5515 | |
| 787 | 0 | |n https://doaj.org/toc/2164-554X | |
| 856 | 4 | 1 | |u https://doaj.org/article/8d5fc53a2a0b4eed9df1510b3e7f37d6 |z Connect to this object online. |