Isolation of Leptospira kmetyi from residential areas of patients with leptospirosis in Kelantan, Malaysia
Background: Environmental sampling provides important information that enhances the understanding of the leptospiral human-environment-animal relationship. Several studies have described the distribution of Leptospira in the environment. However, more targeted sites, that is, areas surrounding lepto...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Book |
| Published: |
Elsevier,
2018-07-01T00:00:00Z.
|
| Subjects: | |
| Online Access: | Connect to this object online. |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Background: Environmental sampling provides important information that enhances the understanding of the leptospiral human-environment-animal relationship. Several studies have described the distribution of Leptospira in the environment. However, more targeted sites, that is, areas surrounding leptospirosis patients' houses, remain under-explored. Therefore, this study aims to detect the presence of Leptospira spp. in the residential areas of patients with leptospirosis. Methods: Soil and water samples near leptospirosis patients' residences were collected, processed and cultured into EMJH media. Partial 16S rRNA gene sequencing was performed to confirm the identity of Leptospira. Results: EMJH culture and partial 16S rRNA gene sequencing revealed predominant growth of pathogenic Leptospira kmetyi (17%, n = 7/42). All tested locations had at least one Leptospira sp., mostly from the soil samples. Conclusion: More than one species of Leptospira may be present in a sampling area. The most common environmental isolates were pathogenic L. kmetyi. Keywords: Leptospirosis, Leptospira, Environment, EMJH, PCR |
|---|---|
| Item Description: | 1876-0341 10.1016/j.jiph.2017.12.008 |