Isolation of Leptospira kmetyi from residential areas of patients with leptospirosis in Kelantan, Malaysia

Background: Environmental sampling provides important information that enhances the understanding of the leptospiral human-environment-animal relationship. Several studies have described the distribution of Leptospira in the environment. However, more targeted sites, that is, areas surrounding lepto...

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Autori principali: Mohammad R. Mohd Ali (Autore), Amira W. Mohamad Safiee (Autore), Nik Y. Yusof (Autore), Mohd H. Fauzi (Autore), Chan Yean Yean (Autore), Nabilah Ismail (Autore)
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Pubblicazione: Elsevier, 2018-07-01T00:00:00Z.
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Riassunto:Background: Environmental sampling provides important information that enhances the understanding of the leptospiral human-environment-animal relationship. Several studies have described the distribution of Leptospira in the environment. However, more targeted sites, that is, areas surrounding leptospirosis patients' houses, remain under-explored. Therefore, this study aims to detect the presence of Leptospira spp. in the residential areas of patients with leptospirosis. Methods: Soil and water samples near leptospirosis patients' residences were collected, processed and cultured into EMJH media. Partial 16S rRNA gene sequencing was performed to confirm the identity of Leptospira. Results: EMJH culture and partial 16S rRNA gene sequencing revealed predominant growth of pathogenic Leptospira kmetyi (17%, n = 7/42). All tested locations had at least one Leptospira sp., mostly from the soil samples. Conclusion: More than one species of Leptospira may be present in a sampling area. The most common environmental isolates were pathogenic L. kmetyi. Keywords: Leptospirosis, Leptospira, Environment, EMJH, PCR
Descrizione del documento:1876-0341
10.1016/j.jiph.2017.12.008