Intratubular Enterococcus faecalis viability assessment following root canal instrumentation with rotary and reciprocating systems via fluorescence microscopy

• Main Authors: Jamileh Ghoddusi (Author), Ehsan Arian (Author), Maryam Golmohammadi (Author), Maryam Gharechahi (Author), Siavash Moushekhian (Author)
• Format: Book
• Published: Tabriz University of Medical Sciences, 2020-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 2008-210X; 2008-2118; 10.34172/joddd.2020.044
• Keywords: antimicrobial test; bacterial viability; enterococcus faecalis; instrumentation; protaper gold system; root canal preparation; waveone systems; Dentistry; RK1-715
• Source: Journal of Dental Research, Dental Clinics, Dental Prospects, Vol 14, Iss 4, Pp 214-217 (2020)

Background. The present in vitro study aimed to compare the effectiveness of the WaveOne and ProTaper Gold systems in removing the Enterococcus faecalis biofilm. Methods. Thirty-eight mandibular premolars were selected. The root canals were assigned to standard control (canals serially enlarged with ProTaper Gold S1-S2-F1-F2, n=15) and experimental (canals enlarged with Primary WaveOne file, n=15) groups. Following the instrumentation procedure, the root canals underwent a sampling procedure, and the colonyforming unit (CFU) counts were determined. The samples were also evaluated under a fluorescent microscope to evaluate viable bacteria. The data were analyzed using independent samples t test and paired samples t test. Results. The results showed that, compared with the ProTaper group, the WaveOne group exhibited the least viable bacteria (P=0.004). Conclusion. It was concluded that comparison with the ProTaper Gold rotary system, the WaveOne reciprocating file is more successful in reducing intratubular viable bacteria counts.