Molecular Detection of Metronidazole and Tetracycline Resistance Genes in <i>Helicobacter pylori</i>-Like Positive Gastric Samples from Pigs
Antimicrobial resistance is a major public health concern. The aim of this study was to assess the presence of antibiotic resistance genes, previously reported in <i>Helicobacter pylori,</i> in gastric samples of 36 pigs, in which DNA of <i>H. pylori</i>-like organisms had be...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Book |
| Published: |
MDPI AG,
2023-05-01T00:00:00Z.
|
| Subjects: | |
| Online Access: | Connect to this object online. |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Antimicrobial resistance is a major public health concern. The aim of this study was to assess the presence of antibiotic resistance genes, previously reported in <i>Helicobacter pylori,</i> in gastric samples of 36 pigs, in which DNA of <i>H. pylori</i>-like organisms had been detected. Based on PCR and sequencing analysis, two samples were positive for the <i>16S rRNA</i> mutation gene, conferring tetracycline resistance, and one sample was positive for the <i>frxA</i> gene with a single nucleotide polymorphism, conferring metronidazole resistance. All three amplicons showed the highest homology with <i>H. pylori</i>-associated antibiotic resistance gene sequences. These findings indicate that acquired antimicrobial resistance may occur in <i>H. pylori</i>-like organisms associated with pigs. |
|---|---|
| Item Description: | 10.3390/antibiotics12050906 2079-6382 |