Effect of compressive force combined with vibration on CCL2 and CCL5 in human periodontal ligament cells

• Main Authors: Supunsa Pongtiwattanakul (Author), Chidchanok Leethanakul (Author), Onnicha Rattanaporn (Author), Peungchaleoy Thammanichanon (Author), Sissada Tannukit (Author)
• Format: Book
• Published: Elsevier, 2024-09-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 2212-4268; 10.1016/j.jobcr.2024.08.005
• Keywords: Accelerated tooth movement; Chemokine; Orthodontic force; Vibration; Dentistry; RK1-715
• Source: Journal of Oral Biology and Craniofacial Research, Vol 14, Iss 5, Pp 626-630 (2024)

Purpose: To investigate the effect of compressive force combined with vibration on expression of CC-chemokine ligand 2 (CCL2) and 5 (CCL5) in human periodontal ligament (hPDL) cells. Methods: Human PDL cells were cultured and assigned into four groups: control (Con), compressive force 2.0 g/cm2 for 24 h and 48 h (C), vibration 0.3 g 30 Hz for 20 min every 24 h (V), and compressive force combined with vibration (VC). At 24 h and 48 h, mRNA and protein levels of CCL2 and CCL5 were examined by quantitative reverse transcription polymerase chain reaction (qRT-PCR) and enzyme-linked immunosorbent assay (ELISA), respectively. Results: At 24 h and 48 h, CCL2 mRNA and protein levels in C and VC were significantly higher than Con. At 24 h, VC showed significantly higher CCL2 mRNA expression than C. However, there was no significant difference between CCL2 protein in C and VC at both time points. At 24 h and 48 h, CCL5 mRNA expression was significantly down-regulated in V and VC, whereas CCL5 protein was undetectable in all groups. Conclusions: Application of compressive force combined with vibration resulted in the upregulation of CCL2 mRNA and protein levels, whereas CCL5 mRNA expression was down-regulated.