A bioorthogonal methylene blue derived probe for targeted photoacoustic imaging

• Main Authors: Afaf R. Genady (Author), Samantha R. Slikboer (Author), Rowan Swann (Author), Kevin Wyszatko (Author), Nancy Janzen (Author), Luis Rafael Silva (Author), Mohamed E. El-Zaria (Author), Saman Sadeghi (Author), John F. Valliant (Author)
• Format: Book
• Published: Elsevier, 2022-04-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

Photoacoustic (PA) imaging is a nonradioactive and noninvasive imaging modality that can volumetrically map the distribution of photo-absorbing molecules with deeper tissue penetration than optical modalities. Here, we report on a convenient strategy to synthesize a modular methylene blue tetrazine (MB-Tz) PA imaging agent and a trans-cyclooctene (TCO)-labeled bisphosphonate (TCO-BP) calcium metabolism targeting vector. PA imaging of MB-Tz pre-clicked to TCO-BP demonstrated significant signal in regions of active bone metabolism (knee and shoulder joints) at 1 ​h post-injection. Targeting validation and whole-body biodistribution studies were carried out with the radiolabeled pre-clicked product of MB-Tz with 99mTc-TCO-BP, confirming uptake in knees and shoulders at 1 ​h post-injection.

Item Description

2772-4174
10.1016/j.ejmcr.2021.100021