Hymenaea rubriflora Ducke stem bark extract has vasorelaxant and contractile inhibition capacity

• Main Authors: Keyth Sulamitta de Lima Guimarães (Author), Luciana Tavares Toscano (Author), Bagnólia Araújo Costa (Author), Iara Leão Luna de Souza (Author), Isabelle de Lima Brito Polari (Author), Ivyne Oliveira Araújo Wanderley (Author), Manoel Miranda Neto (Author), Bárbara Cavalcanti Barros (Author), Rubens Teixeira de Queiroz (Author), Ângela Maria Tribuzy de Magalhães Cordeiro (Author), Maria da Conceição Rodrigues Gonçalves (Author), Lydiane de Lima Tavares Toscano (Author), Alexandre Sérgio Silva (Author)
• Format: Book
• Published: Universidade de São Paulo, 2024-02-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 2175-9790; 10.1590/s2175-97902024e23484
• Keywords: Antioxidant; Aorta; Hymenaea rubriflora ducke; Nitric oxide; Vasorelaxation; Pharmacy and materia medica; RS1-441
• Source: Brazilian Journal of Pharmaceutical Sciences, Vol 60 (2024)

Abstract We investigated the vasodilatory effects of Hymenaea rubriflora Ducke stem bark extract (HR- HAc). Vascular reactivity of the aortic rings of Wistar rats was tested by in vitro cumulative doses (0.1 - 729 μg/mL). Rats (n=5) were treated with 25 (G25), 50 (G50) and 100 (G100) mg/ kg of HR-HAc or saline (control group - CG) for four weeks. An in vitro assay resulted in dose-dependent relaxation of the aortic rings with functional endothelium, which was inhibited in the presence of L-NAME. Rings of the treated animals increased acetylcholine relaxing potency at all doses, with a greater effect on G50 (pD2 = 7.8±0.1, Emax = 95.6±1.1) and a decreased contractile potency to phenylephrine in G25 (pD2 = 6.9±0.06, Emax = 61.5±6.0%) and G50 (pD2= 6.6±0.06, Emax = 71.0±8.5%) when compared to the CG in the presence and absence of endothelium (pD2= 6.4± 0.1, 6.4±0.1 and 6.9±0.1, respectively). Cumulative doses of nitroprusside resulted in increased relaxing potency in all treated groups and maintained Emax at 100%. It is concluded that HR-HAc has vasorelaxant capacity and inhibitory vascular contraction activity applied either directly to aortic rings or after treatment with in vivo supplementation, which places this extract as a potential nutraceutical or pharmacological agent for treating diseases associated with vascular dysfunction.