Pre-maturational culture promotes the developmental competence of bovine oocytes derived from an 8-day in vitro growth culture system

In this study, we evaluated the effects of pre-maturational culture (pre-IVM) on the developmental competence of bovine oocytes derived from an 8-day in vitro growth (IVG) culture system. IVG oocytes were subjected to 5 h pre-IVM before in vitro maturation, followed by in vitro fertilization (IVF)....

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Main Authors: Madalitso CHELENGA (Author), Yojiro YANAGAWA (Author), Seiji KATAGIRI (Author), Masashi NAGANO (Author)
Format: Book
Published: The Society for Reproduction and Development, 2023-05-01T00:00:00Z.
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Summary:In this study, we evaluated the effects of pre-maturational culture (pre-IVM) on the developmental competence of bovine oocytes derived from an 8-day in vitro growth (IVG) culture system. IVG oocytes were subjected to 5 h pre-IVM before in vitro maturation, followed by in vitro fertilization (IVF). The proportion of oocytes that progressed to the germinal vesicle breakdown stage was similar between groups with and without pre-IVM. Although metaphase II oocytes and cleavage rates after IVF were similar regardless of pre-IVM culture, the blastocyst rate was significantly higher in the group with pre-IVM (22.5%) than without pre-IVM (11.0%, P < 0.05). In conclusion, pre-IVM culture improved the developmental competence of bovine oocytes derived from an 8-day IVG system.
Item Description:0916-8818
1348-4400
10.1262/jrd.2023-022