Preparative isolation and puriï¬cation of 12,13-dihydroxyeuparin from Radix Eupatorii Chinensis by high-speed counter-current chromatography
An efï¬cient method for the isolation and puriï¬cation of 12,13-dihydroxyeuparin from Radix Eupatorii Chinensis by high speed counter-current chromatography (HSCCC) was established in this paper. The ether extracts of Radix Eupatorii Chinensis were puriï¬ed by HSCCC with a solvent system of hexyl hy...
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Elsevier,
2012-08-01T00:00:00Z.
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|---|---|---|---|
| 001 | doaj_94c69eea593d4561b6d83a246ca7eefa | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a Mei Yang |e author |
| 700 | 1 | 0 | |a Xin-Jun Xu |e author |
| 700 | 1 | 0 | |a Chun-Yan Xie |e author |
| 700 | 1 | 0 | |a Jie-Yun Huang |e author |
| 700 | 1 | 0 | |a Zhi-Sheng Xie |e author |
| 700 | 1 | 0 | |a De-Po Yang |e author |
| 245 | 0 | 0 | |a Preparative isolation and puriï¬cation of 12,13-dihydroxyeuparin from Radix Eupatorii Chinensis by high-speed counter-current chromatography |
| 260 | |b Elsevier, |c 2012-08-01T00:00:00Z. | ||
| 500 | |a 2095-1779 | ||
| 500 | |a 10.1016/j.jpha.2012.03.004 | ||
| 520 | |a An efï¬cient method for the isolation and puriï¬cation of 12,13-dihydroxyeuparin from Radix Eupatorii Chinensis by high speed counter-current chromatography (HSCCC) was established in this paper. The ether extracts of Radix Eupatorii Chinensis were puriï¬ed by HSCCC with a solvent system of hexyl hydrideâethyl acetateâmethanolâwater (1:2:1:2, v/v/v/v). The upper phase was used as the stationary phase and the lower phase as the mobile phase. About 8.4 mg of 12,13-dihydroxyeuparin was obtained from 200 mg of ether extracts from Radix Eupatorii Chinensis in one-step HSCCC separation, with the purity of 96.71%, as determined by HPLC. After methanolâwater recrystallization, the purity of 12,13-dihydroxyeuparin reached 99.83%. Such a simple and effective method was fairly useful to prepare pure compound as reference substances for related study on Radix Eupatorii Chinensis. Keywords: Radix Eupatorii Chinensis, High speed counter-current chromatography (HSCCC), 12,13-dihydroxyeuparin | ||
| 546 | |a EN | ||
| 690 | |a Therapeutics. Pharmacology | ||
| 690 | |a RM1-950 | ||
| 655 | 7 | |a article |2 local | |
| 786 | 0 | |n Journal of Pharmaceutical Analysis, Vol 2, Iss 4, Pp 258-263 (2012) | |
| 787 | 0 | |n http://www.sciencedirect.com/science/article/pii/S2095177912000445 | |
| 787 | 0 | |n https://doaj.org/toc/2095-1779 | |
| 856 | 4 | 1 | |u https://doaj.org/article/94c69eea593d4561b6d83a246ca7eefa |z Connect to this object online. |