Immunotropic effects of so-called slag metabolites (creatinine, urea, uric acid and bilirubin) at rats

• Main Authors: Igor Popovych (Author), Anatoliy Gozhenko (Author), Igor Kuchma (Author), Walery Zukow (Author), Volodymyra Bilas (Author), Galyna Koval'chuk (Author), Anzhela Ivasivka (Author)
• Format: Book
• Published: Kazimierz Wielki University, 2020-11-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

<p class="MsoNormal" style="text-autospace: ideograph-numeric; mso-pagination: none; text-align: justify; text-justify: inter-ideograph;" align="justify"><strong><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-ansi-font-weight: bold; font-size: 12,0000pt;">Background. </span></strong><span class="15" style="mso-spacerun: 'yes'; font-family: 'Times New Roman';">The study of the effect of end products of protein and nucleic acid metabolism on the immune system is of interest not only theoretical but also practical in line with the problems of urotoxins and hemodialysis.</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;"> </span><span class="15" style="mso-spacerun: 'yes'; font-family: 'Times New Roman';">In previous studies, we found significant links between uricemia and uricosuria, on the one hand, and immune parameters, on the other hand.</span><span class="16" style="mso-spacerun: 'yes'; font-family: 'Times New Roman';"> </span><span class="15" style="mso-spacerun: 'yes'; font-family: 'Times New Roman';">In this study, the spectrum of nitrogenous metabolites was expanded due to creatinine, urea and bilirubin. </span><strong><span class="15" style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-ansi-font-weight: bold;">Material and methods</span></strong><span class="15" style="mso-spacerun: 'yes'; font-family: 'Times New Roman';">. </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">Experiment was performed on </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">60</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;"> healthy female Wistar rats. The plasma level and urinary excretion of the nitrogenous metabolites were determined</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">. </span><span class="15" style="mso-spacerun: 'yes'; font-family: 'Times New Roman';">Immune status was assessed by thymocytogram, splenocytogram, blood leukocytogram and immunocytogram, as well as phagocytosis of blood neutrophils and monocytes.</span><span class="15" style="mso-spacerun: 'yes'; font-family: 'Times New Roman';"> </span><strong><span class="15" style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-ansi-font-weight: bold;">Results</span></strong><span class="15" style="mso-spacerun: 'yes'; font-family: 'Times New Roman';">. </span><span class="15" style="mso-spacerun: 'yes'; font-family: 'Times New Roman';">Both negative and positive metabolic-immune correlations were revealed.</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;"> </span><span class="15" style="mso-spacerun: 'yes'; font-family: 'Times New Roman';">Calculation of multiple correlation coefficients between individual metabolite parameters and constellations of immune parameters revealed the maximum immunotropic effect of uricosuria</span><span class="15" style="mso-spacerun: 'yes'; font-family: 'Times New Roman';"> (R=0,637)</span><span class="15" style="mso-spacerun: 'yes'; font-family: 'Times New Roman';">.</span><span class="16" style="mso-spacerun: 'yes'; font-family: 'Times New Roman';"> </span><span class="15" style="mso-spacerun: 'yes'; font-family: 'Times New Roman';">This is followed by excretion of urea (R=0,617) and creatinine (R=0,606), bilirubinemia (R=0,589), creatinineemia (R=0,567), uricemia (R=0,566) and plasma urea level (R=0,500). The canonical correlation between the constellation of nitrogenous metabolites, on the one hand, and the parameters of immunity, on the other hand, was very strong</span><span class="15" style="mso-spacerun: 'yes'; font-family: 'Times New Roman';">: </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-bidi-font-weight: bold; font-size: 12,0000pt;">R=0,921; χ</span>^{<span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-bidi-font-weight: bold; font-size: 12,0000pt; vertical-align: super;">2</span>}_{<span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-bidi-font-weight: bold; font-size: 12,0000pt; vertical-align: sub;">(154)</span>}<span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-bidi-font-weight: bold; font-size: 12,0000pt;">=282; p<10</span>^{<span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-bidi-font-weight: bold; font-size: 12,0000pt; vertical-align: super;">-6</span>}<span class="15" style="mso-spacerun: 'yes'; font-family: 'Times New Roman';">. </span><strong><span class="15" style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-ansi-font-weight: bold;">Conclusion</span></strong><span class="15" style="mso-spacerun: 'yes'; font-family: 'Times New Roman';">. Nitrogen metabolites exhibit significant immunotropic activity, both suppressor and enhancing.</span></p>

Item Description

2391-8306
10.12775/JEHS.2020.10.11.033