Comparative Investigation of Amino Acids Content in the Dry Extracts of <i>Juno bucharica</i>, <i>Gladiolus Hybrid Zefir</i>, <i>Iris Hungarica,</i> <i>Iris Variegata</i> and <i>Crocus Sativus</i> Raw Materials of Ukrainian Flora

• Main Authors: Olha Mykhailenko (Author), Liudas Ivanauskas (Author), Ivan Bezruk (Author), Roman Lesyk (Author), Victoriya Georgiyants (Author)
• Format: Book
• Published: MDPI AG, 2020-02-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

The aim of this research was the comparative study of the amino acids content in the dry extracts of <i>Iridaceae</i> plants of Ukrainian flora: <i>Juno bucharica</i> leaves and corms, <i>Gladiolus hybrid Zefir</i> leaves, <i>Iris hungarica</i> and <i>Iris variegata</i> rhizomes, and <i>Crocus sativus</i> stigmas, flowers, leaves and corms. A gas chromatography&#8722;mass spectrometry (GC&#8722;MS) method has been used. Separation of amino acids in the samples was carried out using a Shimadzu GC-MS-QP2010 equipped with an Rxi-5ms (Restek Corporation capillary column (30 m long, 0.25 mm outer diameter and 0.25 &#181;m) with a liquid stationary phase (5% diphenyl and 95% polysiloxane) after derivatization with N-(t-butyldimethylsilyl)-N109 methyltrifluoroacetamide (MTBSTFA) reagent. The results obtained have shown that extracts from the aerial parts of plants investigated have a higher amino acid content and more diverse composition than the underground organs. Experimental data showed that <i>Crocus</i> leaves and <i>Juno</i> leaves extracts contain the highest general content of amino acids&#8212;31.99 mg/g and 14.65 mg/g respectively. All samples showed a high content of L-pyroglutamic acid (0.33&#8722;12.35 mg/g). Moreover, <i>Crocus</i> leaves and <i>Juno</i> leaves extracts had the most suitable amino acids composition and are prospective for further pharmacological studies.

Item Description

2218-0532
10.3390/scipharm88010008