Salivary Metabolites in Patients with Mucopolysaccharidosis

• Main Authors: Rafaela de Oliveira Torres (Author), Andréa Vaz Braga Pintor (Author), Tatiana Kelly da Silva Fidalgo (Author), Ana Paula Canedo Valente (Author), Liana Bastos Freitas-Fernandes (Author), Ivete Pomarico Ribeiro de Souza (Author)
• Format: Book
• Published: Association of Support to Oral Health Research (APESB), 2023-11-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

Objective: To identify the salivary metabolites profile of Mucopolysaccharidosis (MPS) types I, II, IV, and VI patients. Material and Methods:The participants were asked to refrain from eating and drinking for one hour before sampling, performed between 7:30 and 9:00 a.m. Samples were centrifuged at 10.000 × g for 60 min at 4ºC, and the supernatants (500µl) were stored at -80ºC until NMR analysis. The salivary proton nuclear magnetic resonance (1H-NMR) spectra were acquired in a 500 MHz spectrometer, and TOCSY experiments were used to confirm and assign metabolites. Data were analyzed descriptively. Results: Differences in salivary metabolites were found among MPS types and the control, such as lactate, propionate, alanine, and N-acetyl sugar. Understanding these metabolite changes may contribute to precision medicine and early detection of mucopolysaccharidosis and its monitoring. Conclusion: The composition of low molecular weight salivary metabolites of mucopolysaccharidosis subjects may present specific features compared to healthy controls.

Item Description

1519-0501
1983-4632