Validation of automated positive cell and region detection of immunohistochemically stained laryngeal tumor tissue using digital image analysis

• Egile Nagusiak: Hilde J.G. Smits (Egilea), Justin E. Swartz (Egilea), Marielle E.P. Philippens (Egilea), Remco de Bree (Egilea), Johannes H.A.M. Kaanders (Egilea), Sjors A. Koppes (Egilea), Gerben E. Breimer (Egilea), Stefan M. Willems (Egilea)
• Formatua: Liburua
• Argitaratua: Elsevier, 2023-01-01T00:00:00Z.
• Gaiak: article
• Sarrera elektronikoa: Connect to this object online.

Objectives: This study aimed to validate a digital image analysis (DIA) workflow for automatic positive cell detection and positive region delineation for immunohistochemical hypoxia markers with a nuclear (hypoxia-inducible factor 1α [HIF-1α]) and a cytoplasmic (pimonidazole [PIMO]) staining patter...