Emergence of High-Level Gentamicin Resistance in <i>Streptococcus agalactiae</i> Hypervirulent Serotype IV ST1010 (CC452) Strains by Acquisition of a Novel Integrative and Conjugative Element
<i>Streptococcus agalactiae</i> (group B streptococci, GBS) is responsible for severe infections in both neonates and adults. Currently, empiric antimicrobial therapy for sepsis and meningitis is the combined use of penicillin and gentamicin due to the enhanced bactericidal activity. How...
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MDPI AG,
2024-05-01T00:00:00Z.
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| LEADER | 00000 am a22000003u 4500 | ||
|---|---|---|---|
| 001 | doaj_971037e0cf0b4c74b32ba56bd1a00d06 | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a Roberta Creti |e author |
| 700 | 1 | 0 | |a Monica Imperi |e author |
| 700 | 1 | 0 | |a Uzma Basit Khan |e author |
| 700 | 1 | 0 | |a Alberto Berardi |e author |
| 700 | 1 | 0 | |a Simona Recchia |e author |
| 700 | 1 | 0 | |a Giovanna Alfarone |e author |
| 700 | 1 | 0 | |a Giovanni Gherardi |e author |
| 245 | 0 | 0 | |a Emergence of High-Level Gentamicin Resistance in <i>Streptococcus agalactiae</i> Hypervirulent Serotype IV ST1010 (CC452) Strains by Acquisition of a Novel Integrative and Conjugative Element |
| 260 | |b MDPI AG, |c 2024-05-01T00:00:00Z. | ||
| 500 | |a 10.3390/antibiotics13060491 | ||
| 500 | |a 2079-6382 | ||
| 520 | |a <i>Streptococcus agalactiae</i> (group B streptococci, GBS) is responsible for severe infections in both neonates and adults. Currently, empiric antimicrobial therapy for sepsis and meningitis is the combined use of penicillin and gentamicin due to the enhanced bactericidal activity. However, high-level gentamicin resistance (HLGR) abrogates the synergism. The rate of HLGR was investigated within a dataset of 433 GBS strains collected from cases of invasive disease in both adults and neonates as well as from pregnant carriers. GBS isolates (n = 20, 4.6%) presented with HLGR (gentamicin MIC breakpoint >1024 mg/L) that was differently diffused between strains from adults or neonates (5.2% vs. 2.8%). Notably, 70% of HLGR GBS strains (14 isolates) were serotype IV. Serotype IV HLGR-GBS isolates were susceptible to all antibiotics tested, exhibited the alpha-C/HvgA/PI-2b virulence string, and belonged to sequence type 1010 (clonal complex (CC) 452). The mobile element that harbored the HLGR <i>aac</i>(6')-<i>aph</i>(2)" gene is a novel integrative and conjugative element (ICE) about 45 kb long, derived from GBS 515 ICE tRNA<sup><i>Lys</i></sup>. The clonal expansion of this HLGR hypervirulent serotype IV GBS CC452 sublineage may pose a threat to the management of infections caused by this strain type. | ||
| 546 | |a EN | ||
| 690 | |a <i>Streptococcus agalactiae</i> | ||
| 690 | |a GBS | ||
| 690 | |a high-level gentamicin resistance (HLGR) | ||
| 690 | |a ST1010 | ||
| 690 | |a serotype IV | ||
| 690 | |a CC452 | ||
| 690 | |a Therapeutics. Pharmacology | ||
| 690 | |a RM1-950 | ||
| 655 | 7 | |a article |2 local | |
| 786 | 0 | |n Antibiotics, Vol 13, Iss 6, p 491 (2024) | |
| 787 | 0 | |n https://www.mdpi.com/2079-6382/13/6/491 | |
| 787 | 0 | |n https://doaj.org/toc/2079-6382 | |
| 856 | 4 | 1 | |u https://doaj.org/article/971037e0cf0b4c74b32ba56bd1a00d06 |z Connect to this object online. |