Genomic Characterization of <i>Salmonella</i> Isangi: A Global Perspective of a Rare Serovar

• Main Authors: Anamaria Mota Pereira dos Santos (Author), Pedro Panzenhagen (Author), Rafaela G. Ferrari (Author), Ana Carolina S. de Jesus (Author), Ana Beatriz Portes (Author), Alan Clavelland Ochioni (Author), Dalia dos Prazeres Rodrigues (Author), Carlos Adam Conte-Junior (Author)
• Formato: Livro
• Publicado em: MDPI AG, 2023-08-01T00:00:00Z.
• Assuntos: article
• Acesso em linha: Connect to this object online.
• Notes: 10.3390/antibiotics12081309; 2079-6382
• Keywords: whole-genome sequencing; nontyphoidal <i>Salmonella</i>; multidrug resistance; ESBL; PMQR; quinolone; Therapeutics. Pharmacology; RM1-950
• Source: Antibiotics, Vol 12, Iss 8, p 1309 (2023)

<i>Salmonella</i> Isangi is an infrequent serovar that has recently been reported in several countries due to nosocomial infections. A considerable number of reports indicate <i>Salmonella</i> Isangi multidrug resistance, especially to cephalosporins, which could potentially pose a risk to public health worldwide. Genomic analysis is an excellent tool for monitoring the emergence of microorganisms and related factors. In this context, the aim of this study was to carry out a genomic analysis of <i>Salmonella</i> Isangi isolated from poultry in Brazil, and to compare it with the available genomes from the Pathogen Detection database and Sequence Read Archive. A total of 142 genomes isolated from 11 different countries were investigated. A broad distribution of extended-spectrum beta-lactamase (ESBL) genes was identified in the <i>Salmonella</i> Isangi genomes examined (<i>bla</i>_CTX-M-15, <i>bla</i>_CTX-M-2, <i>bla</i>_DHA-1, <i>bla</i>_NDM-<i>1</i>, <i>bla</i>_OXA-10, <i>bla</i>_OXA-1, <i>bla</i>_OXA-48, <i>bla</i>_SCO-1, <i>bla</i>_SHV-<i>5</i>, <i>bla</i>_TEM-131, <i>bla</i>_TEM-1B), primarily in South Africa. Resistome analysis revealed predicted resistance to aminoglycoside, sulfonamide, macrolide, tetracycline, trimethoprim, phenicol, chloramphenicol, and quaternary ammonium. Additionally, PMQR (plasmid-mediated quinolone resistance) genes <i>qnr19</i>, <i>qnrB1</i>, and <i>qnrS1</i> were identified, along with point mutations in the genes <i>gyrA</i>^D87N, <i>gyrA</i>^S83F, and <i>gyrB</i>^S464F, which confer resistance to ciprofloxacin and nalidixic acid. With regard to plasmids, we identified 17 different incompatibility groups, including IncC, Col(pHAD28), IncHI2, IncHI2A, IncM2, ColpVC, Col(Ye4449), Col156, IncR, IncI1(Alpha), IncFIB (pTU3), Col(B5512), IncQ1, IncL, IncN, IncFIB(pHCM2), and IncFIB (pN55391). Phylogenetic analysis revealed five clusters grouped by sequence type and antimicrobial gene distribution. The study highlights the need for monitoring rare serovars that may become emergent due to multidrug resistance.