Characterization of therapeutic proteins by cation exchange chromatography-mass spectrometry and top-down analysis

• Main Authors: Rachel Liuqing Shi (Author), Gang Xiao (Author), Thomas M. Dillon (Author), Margaret S. Ricci (Author), Pavel V. Bondarenko (Author)
• Format: Book
• Published: Taylor & Francis Group, 2020-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Recently, cation exchange chromatography (CEX) using aqueous volatile buffers was directly coupled with mass spectrometry (MS) and applied for intact analysis of therapeutic proteins and antibodies. In our study, chemical modifications responsible for charge variants were identified by CEX-UV-MS for...