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Effect of a Dihydrobenzofuran Derivative on Lipid Hydroperoxide-Induced Rabbit Corneal Neovascularization

The aim of this study was to investigate the effect of A-3922, a dihydrobenzofuran derivative, on linoleic acid hydroperoxide (LHP)-induced corneal neovascularization (NV) in a rabbit model. Male New Zealand rabbits Received intraperitoneal (i.p.) injections of 10 or 30 mg/kg per day A-3922 or its v...

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Main Authors: Hirotsugu Ogura (Author), Takako Nakanishi-Ueda (Author), Toshihiko Ueda (Author), Shinichi Iwai (Author), Seiichi Uchida (Author), Yuta Saito (Author), Yoko Taguchi (Author), Hajime Yasuhara (Author), Donald Armstrong (Author), Katsuji Oguchi (Author), Ryohei Koide (Author)
Format: Book
Published: Elsevier, 2007-01-01T00:00:00Z.
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Summary:The aim of this study was to investigate the effect of A-3922, a dihydrobenzofuran derivative, on linoleic acid hydroperoxide (LHP)-induced corneal neovascularization (NV) in a rabbit model. Male New Zealand rabbits Received intraperitoneal (i.p.) injections of 10 or 30 mg/kg per day A-3922 or its vehicle as control for 3 days. One day after i.p. injections, LHP was injected with a 30-gauge needle into the corneal stroma of the superior quadrant 4.5-mm below the limbus. Photographs of the vessels were taken for digital analysis with a surgical microscope. Vascular endothelial growth factor (VEGF) was measured using an immunoassay kit, and matrix metalloproteinase (MMP)-9 was measured by gelatin zymography in corneal samples. At 7 days post-LHP injection, the total vessel length was 26.7 ± 3.8 mm in the control animals (n = 8), 16.1 ± 0.8 mm in the A-3922 (10 mg/kg)-treated group (n = 5), and 11.4 ± 2.1 mm in the 30 mg/kg group (n = 8, P<0.01 vs control), respectively. After LHP injection, the content of VEGF and MMP-9 activity were increased in the superior cornea, but these were not influenced by A-3922 treatments. These results indicate that LHP-induced corneal NV is inhibited by treatment with A-3922 and therefore may represent a potential pharmacological intervention for ocular neovascularization disorders. Keywords:: neovascularization, lipid hydroperoxide, dihydrobenzofuran derivative, vascular endothelial growth factor (VEGF), matrix metalloproteinase (MMP)
Item Description:1347-8613
10.1254/jphs.FP0061301

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