The influence of the metabolic activity of the intestinal microflora on the severity and duration of rotavirus diarrhea in early-aged children

• Main Authors: N. V. Vorobiova (Author), O. V. Usachova (Author), Ye. A. Silina (Author), O. O. Furyk (Author)
• Format: Book
• Published: Zaporozhye State Medical University, 2023-04-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 10.14739/2310-1237.2023.1.272745; 2306-8027; 2310-1237
• Keywords: viral infection; infectious diarrhea; children; early age; intestinal microflora; short-chain fatty acids; risk factors; prognosis; Pathology; RB1-214
• Source: Patologìâ, Vol 20, Iss 1, Pp 63-68 (2023)

The aim is to determine the pathogenetic role of the metabolic activity of the intestinal microflora in the formation of the severity and duration of rotavirus diarrhea in early-aged children. Materials and methods. 60 children aged 1-24 months with RVI were included in the research group. The metabolic activity of the intestinal microflora was determined by detecting short-chain fatty acids (SCFAs) in feces in the dynamics of the disease (the absolute concentration (μmol/l) of acetate, propionate, butyrate, the total concentration of SCFAs and the value of the anaerobic index (AI) on the 3rd, 5th and 10th days of RVI) using high-performance liquid chromatography with LC-MS chromatography Agilent 1260 Infinity HPLC System, USA. Results. From the 5th day of RVI, with a minimally expressed diarrheal syndrome, a higher total pool of SCFA and higher AI values were observed than with moderate-severe and severe diarrhea (p ˂ 0.05 on the 5th and 10th days of RVI). A longer duration of the diarrheal syndrome in children was associated with a decrease in the metabolic activity of sucrolytic intestinal bacteria: patients with diarrhea ≤5 days had 2.4 times higher indicators of the total pool of SCFA, than patients with the duration of diarrhea ≥6 days (p ˂ 0.05). The degree of reduction in the concentration of C3 and the value of AI on the 2-3rd days of RVI was directly correlated with the duration of diarrhea, which increased by 1 day with a decrease in C3 by 20.6 μmol/l or a decrease in AI by 0.05. Conclusions. The influence of the intestinal microflora metabolic deficiency on the severity of diarrhea in early-aged children manifests itself from the 5th day of illness. A decrease in the concentration of propionate and the value of AI on the 2-3rd days of the disease is the earliest indicator of prolonged diarrhea in children with RVI.