Features of immune status in different states of uric acid metabolism in female rats

• Main Authors: Anatoliy Gozhenko (Author), Vadym Smagliy (Author), Inna Korda (Author), Nataliya Badiuk (Author), Walery Zukow (Author), Igor Popovych (Author)
• Format: Book
• Published: Kazimierz Wielki University, 2019-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

<p class="pre" style="text-align: justify; text-justify: inter-ideograph; background: #f8f9fa;" align="justify"><strong><span style="font-family: 'Times New Roman';">Background.</span></strong><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 10,0000pt;"> </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">Previously, we </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; color: #222222; font-size: 12,0000pt;">found a wide range of uric acid metabolism parameters</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; color: #222222; font-size: 12,0000pt;"> </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; color: #222222; font-size: 12,0000pt;">and </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">functional relationships of uricemia and uricosuria with the parameters of immunity in </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; color: #222222; font-size: 12,0000pt;">healthy</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;"> female rats analyzed.</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; color: #222222; font-size: 12,0000pt;"> It was found by canonical correlation analysis that both parameters of uric acid metabolism determine the immunity status of rats by 71%</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; color: #222222; font-size: 12,0000pt;">.</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; color: #222222; font-size: 12,0000pt;"> </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; color: #222222; font-size: 12,0000pt;">The purpose of this study is to determine the features of immune status in rats with quantitatively and qualitatively different uric acid metabolism.</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;"> </span><strong><span style="font-family: 'Times New Roman';">Material and Methods</span></strong><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">. Experiment was performed on 60 healthy female Wistar rats 220-300 g. Among them 10 animals remained intact, using tap water from drinking ad libitum. The rats of others groups for 6 days administered through the tube various fluids. The serum and urine levels of the uric acid (uricase method) were determined</span><span class="15" style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; color: #222222; font-size: 12,0000pt; background: #ffffff; mso-shading: #ffffff;">. </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">In the blood, the parameters of immun</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">ity</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;"> were determined</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">. From thymus and spleen made smears-imprints for counting splenocytogram and thymocytogram. For them, as well as immunocytogram and leukocytogram of blood, Shannon's entropy was calculated.</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; color: #222222; font-size: 12,0000pt;"> </span><strong><span style="font-family: 'Times New Roman'; color: #222222;">Results.</span></strong><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; color: #222222; font-size: 12,0000pt;"> </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; color: #222222; font-size: 12,0000pt;">Screening of immune profiles of four quantitative and qualitative uric acid metabolism clusters revealed 6 functional-metabolic patterns, two of which are quasilinear (</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; color: #222222; font-size: 12,0000pt;">enhanc</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; color: #222222; font-size: 12,0000pt;">ing and suppressing), with immune extremes at moderately elevated uricemia, two U-shaped with immune optimum at moderately reduced uricemia levels, as well as a non-reactive pattern. Discriminant analysis revealed 14 parameters of immunity, the constellation of which four clusters of metabolism of uric acid clearly distinguish. </span><strong><span style="font-family: 'Times New Roman'; color: #222222;">Conclusion</span></strong><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; color: #222222; font-size: 12,0000pt;">.</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 10,0000pt;"> </span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">Endogenous</span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;"> </span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">uric</span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;"> </span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">acid</span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;"> </span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">exerts</span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;"> </span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">a</span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;"> </span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">modulatory</span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;"> </span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">immunotropic</span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial;
background-repeat: i
nitial; background-attachment: initial; background-origin: initial; background-clip: initial;"> </span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">effect</span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;"> </span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">in</span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;"> </span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">healthy</span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;"> </span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">female</span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;"> </span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">rats</span><span style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">.</span></p>

Item Description

2391-8306
10.12775/JEHS.2019.09.12.019