Extracellular Reactive Oxygen Species (ROS) Production in Fresh Donkey Sperm Exposed to Reductive Stress, Oxidative Stress and NETosis

Jenny shows a large endometrial reaction after semen influx to the uterus with a large amount of polymorphonuclear neutrophils (PMN) migrating into the uterine lumen. PMN act as a sperm selection mechanism through phagocytosis and NETosis (DNA extrudes and, together with proteins, trap spermatozoa)....

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Main Authors: Iván Yánez-Ortiz (Author), Jaime Catalán (Author), Yentel Mateo-Otero (Author), Marta Dordas-Perpinyà (Author), Sabrina Gacem (Author), Natalia Yeste (Author), Anna Bassols (Author), Marc Yeste (Author), Jordi Miró (Author)
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Published: MDPI AG, 2021-08-01T00:00:00Z.
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LEADER 00000 am a22000003u 4500
001 doaj_9eb2d3c5e5c348f7897c4af067d19cc2
042 |a dc 
100 1 0 |a Iván Yánez-Ortiz  |e author 
700 1 0 |a Jaime Catalán  |e author 
700 1 0 |a Yentel Mateo-Otero  |e author 
700 1 0 |a Marta Dordas-Perpinyà  |e author 
700 1 0 |a Sabrina Gacem  |e author 
700 1 0 |a Natalia Yeste  |e author 
700 1 0 |a Anna Bassols  |e author 
700 1 0 |a Marc Yeste  |e author 
700 1 0 |a Jordi Miró  |e author 
245 0 0 |a Extracellular Reactive Oxygen Species (ROS) Production in Fresh Donkey Sperm Exposed to Reductive Stress, Oxidative Stress and NETosis 
260 |b MDPI AG,   |c 2021-08-01T00:00:00Z. 
500 |a 10.3390/antiox10091367 
500 |a 2076-3921 
520 |a Jenny shows a large endometrial reaction after semen influx to the uterus with a large amount of polymorphonuclear neutrophils (PMN) migrating into the uterine lumen. PMN act as a sperm selection mechanism through phagocytosis and NETosis (DNA extrudes and, together with proteins, trap spermatozoa). While a reduced percentage of spermatozoa are phagocytosed by PMN, most are found to be attached to neutrophil extracellular traps (NETs). This selection process together with sperm metabolism produces a large amount of reactive oxygen species (ROS) that influence the reproductive success. The present study aimed to determine the extracellular ROS production in both sperm and PMN. With this purpose, (1) donkey sperm were exposed to reductive and oxidative stresses, through adding different concentrations of reduced glutathione (GSH) and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), respectively; and (2) PMN were subjected to NETosis in the presence of the whole semen, sperm, seminal plasma (SP) or other activators such as formyl-methionyl-leucyl-phenylalanine (FMLP). Extracellular ROS production (measured as H<sub>2</sub>O<sub>2</sub> levels) was determined with the Amplex<sup>®</sup> Red Hydrogen Peroxide/Peroxidase Assay Kit. Donkey sperm showed more resilience to oxidative stress than to the reductive one, and GSH treatments led to greater H<sub>2</sub>O<sub>2</sub> extracellular production. Moreover, not only did SP appear to be the main inducer of NETosis in PMN, but it was also able to maintain the extracellular H<sub>2</sub>O<sub>2</sub> levels produced by sperm and NETosis. 
546 |a EN 
690 |a reactive oxygen species (ROS) 
690 |a reduced glutathione (GSH) 
690 |a hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) 
690 |a polymorphonuclear neutrophils (PMN) 
690 |a NETosis 
690 |a seminal plasma (SP) 
690 |a Therapeutics. Pharmacology 
690 |a RM1-950 
655 7 |a article  |2 local 
786 0 |n Antioxidants, Vol 10, Iss 9, p 1367 (2021) 
787 0 |n https://www.mdpi.com/2076-3921/10/9/1367 
787 0 |n https://doaj.org/toc/2076-3921 
856 4 1 |u https://doaj.org/article/9eb2d3c5e5c348f7897c4af067d19cc2  |z Connect to this object online.