<i>Anoectochilus roxburghii</i> Extract Extends the Lifespan of <i>Caenorhabditis elegans</i> through Activating the <i>daf-16</i>/FoxO Pathway
As a significant global issue, aging is prompting people's interest in the potential anti-aging properties of <i>Anoectochilus roxburghii</i> (<i>A. roxburghii</i>), a plant traditionally utilized in various Asian countries for its purported benefits in treating diabetes...
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MDPI AG,
2024-08-01T00:00:00Z.
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| Резюме: | As a significant global issue, aging is prompting people's interest in the potential anti-aging properties of <i>Anoectochilus roxburghii</i> (<i>A. roxburghii</i>), a plant traditionally utilized in various Asian countries for its purported benefits in treating diabetes and combating aging. However, the specific anti-aging components and mechanisms of <i>A. roxburghii</i> remain unclear. This study aims to investigate the anti-aging effects and mechanisms of <i>A. roxburghii</i> extract E (ARE). <i>Caenorhabditis elegans</i> (<i>C. elegans</i>) were exposed to media containing different concentrations of ARE whose superior in vitro radical scavenging capacity was thus identified. Lifespan assays, stress resistance tests, and RT-qPCR analyses were conducted to evaluate anti-aging efficacy, reactive oxygen species (ROS) levels, antioxidant enzyme activity, and <i>daf-16</i>, <i>sod-3</i>, and <i>gst-4</i> levels. Additionally, transcriptomic and metabolomic analyses were performed to elucidate the potential anti-aging mechanisms of ARE. Fluorescence protein assays and gene knockout experiments were employed to validate the impacts of ARE on anti-aging mechanisms. Our results revealed that ARE not only prolonged the lifespan of <i>C. elegans</i> but also mitigated ROS and lipofuscin accumulation, and boosted resistance to UV and heat stress. Furthermore, ARE modulated the expression of pivotal anti-aging genes including <i>daf-16</i>, <i>sod-3</i>, and <i>gst-4</i>, facilitating the nuclear translocation of DAF-16. Significantly, ARE failed to extend the lifespan of <i>daf-16</i>-deficient <i>C. elegans</i> (CF1038), indicating its dependency on the <i>daf-16</i>/FoxO signaling pathway. These results underscored the effectiveness of ARE as a natural agent for enhancing longevity and stress resilience to <i>C. elegans</i>, potentially to human. |
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| Опис примірника: | 10.3390/antiox13080945 2076-3921 |