PARAMETERS OF ANTIVIRAL IMMUNITY IN NEWBORNS WITH CYTOMEGALOVIRUS INFECTION IN CASES OF LATE DETECTION OF CYTOMEGALOVIRUS DNA

• Autore principale: L. V. Kravchenko (Autore)
• Natura: Libro
• Pubblicazione: LLC "Diagnostics and Vaccines", 2018-09-01T00:00:00Z.
• Soggetti: article
• Accesso online: Connect to this object online.
• Notes: 2072-8107; 10.22627/2072-8107-2018-17-3-34-37
• Keywords: newborns; cytomegalovirus infection (cmvi); interferons; Pediatrics; RJ1-570
• Source: Детские инфекции (Москва), Vol 17, Iss 3, Pp 34-37 (2018)

Objective: study of immune status indices in newborns in cases of late detection of cytomegalovirus (CMV) DNA in blood and urine.Methods: 147 newborns with non-specific clinical symptoms were studied. Тyping of lymphocytes relating to different clusters CD3+, CD69+, CD71+, CD95+ using monoclonal antibodies produced by IMMUNOTECH (France) have been performed. Expression of membrane markers of immunocompetent cells was measured on a laser flow cytofluorometer Beckman COULTER Epics XL II.Results: In 123 newborns CMVI has been confirmed by the positive result of PCR, while in 24 newborns the negative result was obtained. In 24 three-month old babies, who had a negative result following DNA diagnostics in the first month of their life, cytomegalovirus in blood and urine and increase of anti-CMV IgG have been found, which allowed to diagnose CMVI.The following formula reflecting the dependence of CMVI on CD69+ and CD95+ content in newborns having non-specific clinical symptoms in cases of late detection of CMV DNA has been offered.