Hepatoprotective Effect of Carob Pulp Flour (<i>Ceratonia siliqua</i> L.) Extract Obtained by Optimized Microwave-Assisted Extraction

To examine antioxidant capacity and the hepatoprotective effect of carob pulp flour, microwave-assisted extraction was performed. The influence of ethanol concentration (0-40% <i>w</i>/<i>w</i>), extraction time (5-25 min) and irradiation power (400-800 W) on DPPH, FRAP and A...

Full description

Saved in:
Bibliographic Details
Main Authors: Nikola Martić (Author), Jana Zahorec (Author), Nebojša Stilinović (Author), Bojana Andrejić-Višnjić (Author), Branimir Pavlić (Author), Nebojša Kladar (Author), Dragana Šoronja-Simović (Author), Zita Šereš (Author), Miodrag Vujčić (Author), Olga Horvat (Author), Aleksandar Rašković (Author)
Format: Book
Published: MDPI AG, 2022-03-01T00:00:00Z.
Subjects:
Online Access:Connect to this object online.
Tags: Add Tag
No Tags, Be the first to tag this record!

MARC

LEADER 00000 am a22000003u 4500
001 doaj_a27bf75dacd34a49b3568770a1b6aa3f
042 |a dc 
100 1 0 |a Nikola Martić  |e author 
700 1 0 |a Jana Zahorec  |e author 
700 1 0 |a Nebojša Stilinović  |e author 
700 1 0 |a Bojana Andrejić-Višnjić  |e author 
700 1 0 |a Branimir Pavlić  |e author 
700 1 0 |a Nebojša Kladar  |e author 
700 1 0 |a Dragana Šoronja-Simović  |e author 
700 1 0 |a Zita Šereš  |e author 
700 1 0 |a Miodrag Vujčić  |e author 
700 1 0 |a Olga Horvat  |e author 
700 1 0 |a Aleksandar Rašković  |e author 
245 0 0 |a Hepatoprotective Effect of Carob Pulp Flour (<i>Ceratonia siliqua</i> L.) Extract Obtained by Optimized Microwave-Assisted Extraction 
260 |b MDPI AG,   |c 2022-03-01T00:00:00Z. 
500 |a 10.3390/pharmaceutics14030657 
500 |a 1999-4923 
520 |a To examine antioxidant capacity and the hepatoprotective effect of carob pulp flour, microwave-assisted extraction was performed. The influence of ethanol concentration (0-40% <i>w</i>/<i>w</i>), extraction time (5-25 min) and irradiation power (400-800 W) on DPPH, FRAP and ABTS antioxidant activity of carob pulp flour extract was evaluated. The strongest influence was that of the ethanol concentration, followed by extraction time. Optimal process parameters for maximizing total antioxidant activity were determined, using response surface methodology: ethanol concentration 40%, time 25 min and power 800 W. Carob extract obtained at optimal conditions (CE) was analyzed in vivo using a paracetamol-induced hepatotoxicity model in mice. Treatment with CE attenuated the parameters of liver injury, especially aspartate and alanine aminotransferase activity, and prevented paracetamol-induced increase in malondialdehyde levels. Pretreatment with CE reversed the activities of superoxide dismutase, catalase, glutathione peroxidase and glutathione S-transferase enzymes after the high dose of paracetamol in the liver. Hepatotoxicity induced using a toxic dose of paracetamol was also seen through histopathological alterations, which were significantly reduced in the groups treated with CE prior to paracetamol. Still, the number of Kupffer cells and macrophages did not differ among groups. Finally, pretreatment of mice with CE and paracetamol significantly decreased the expression of cytochrome P450 2E1 (CYP2E1) in hepatocytes. 
546 |a EN 
690 |a carob pulp flour 
690 |a microwave-assisted extraction 
690 |a antioxidant activity 
690 |a response surface methodology 
690 |a paracetamol-induced hepatotoxicity 
690 |a CYP2E1 
690 |a Pharmacy and materia medica 
690 |a RS1-441 
655 7 |a article  |2 local 
786 0 |n Pharmaceutics, Vol 14, Iss 3, p 657 (2022) 
787 0 |n https://www.mdpi.com/1999-4923/14/3/657 
787 0 |n https://doaj.org/toc/1999-4923 
856 4 1 |u https://doaj.org/article/a27bf75dacd34a49b3568770a1b6aa3f  |z Connect to this object online.