Evaluation of Chronic Hepatitis B Infection in Patients With Seronegative HbsAg

• Main Authors: N Sadeghifard (Author), E Gholami Parizad (Author), A Khosravi (Author), S Ghafourian (Author)
• Format: Book
• Published: Tehran University of Medical Sciences, 2012-02-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 2251-6085
• Keywords: Hepatitis B; HBsAg; Serum; Carrier; Ear Cerumen; Public aspects of medicine; RA1-1270
• Source: Iranian Journal of Public Health, Vol 41, Iss 2, Pp 100-104 (2012)

Background: It is estimated that about 370 million people are chronic carriers of HBV worldwide. Apparently 3% of Iranian populations are chronic carriers of this virus. We aimed to evaluate the viral DNA in biological fluids of chronic hepatitis patients compared to a control group.Methods: The current case-control study was designed to evaluate the viral DNA in biological fluids of 70 chronic hepatitis patients compared to a control group using ELISA, PCR and Real Time.Results: All individuals (100%) in case group were HBsAg positive while in control group only 2 individuals (2.8%) were HBsAg positive. Three individuals , in control group were positive using PCR and Real Time PCR indicating that about 7% of those in control group were chronic carriers of HBV. The interesting point was the copy of viral DNA; (5.49 ×104, 2.162×103and 7.26×106) for 3 chronic carriers using sera while it was about (5.71×103, 1.45×102 and 2.56×105) using ear cerumen confirming the necessity of investigating for the carriers of HBV in different biological fluid and by different methods.Conclusion: It can be concluded chronic carriers of hepatitis B are much more than what is diagnosed by routine diagnostic tests. On the other hand ELISA alone can not be relied on as a complete test for screening of chronic carriers in hepatitis B. PCR and Real Time PCR are more reliable tests for this purpose.