Identification of novel biomarkers for neonatal hypoxic-ischemic encephalopathy using iTRAQ

Abstract Background A prompt diagnosis of HIE remains a challenge clinically. This study aimed to identify potential biomarkers of neonatal hypoxic-ischemic encephalopathy (HIE) via a novel proteomic approach, the isobaric tags for absolute and relative quantification (iTRAQ) method. Methods Blood s...

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Main Authors: Yuanyuan Zhu (Author), Yajing Yun (Author), Meifang Jin (Author), Gen Li (Author), Hong Li (Author), Po Miao (Author), Xin Ding (Author), Xing Feng (Author), Lixiao Xu (Author), Bin Sun (Author)
Format: Book
Published: BMC, 2020-05-01T00:00:00Z.
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001 doaj_a43a87c69c1e4d3b8f3b728d0338a104
042 |a dc 
100 1 0 |a Yuanyuan Zhu  |e author 
700 1 0 |a Yajing Yun  |e author 
700 1 0 |a Meifang Jin  |e author 
700 1 0 |a Gen Li  |e author 
700 1 0 |a Hong Li  |e author 
700 1 0 |a Po Miao  |e author 
700 1 0 |a Xin Ding  |e author 
700 1 0 |a Xing Feng  |e author 
700 1 0 |a Lixiao Xu  |e author 
700 1 0 |a Bin Sun  |e author 
245 0 0 |a Identification of novel biomarkers for neonatal hypoxic-ischemic encephalopathy using iTRAQ 
260 |b BMC,   |c 2020-05-01T00:00:00Z. 
500 |a 10.1186/s13052-020-00822-7 
500 |a 1824-7288 
520 |a Abstract Background A prompt diagnosis of HIE remains a challenge clinically. This study aimed to identify potential biomarkers of neonatal hypoxic-ischemic encephalopathy (HIE) via a novel proteomic approach, the isobaric tags for absolute and relative quantification (iTRAQ) method. Methods Blood samples were collected from neonates with mild (n = 4), moderate (n = 4), or severe (n = 4) HIE who were admitted to the neonatal intensive care unit of Children's Hospital of Soochow University between Oct 2015 and Oct 2017. iTRAQ was performed in HIE patients and healthy controls (n = 4). Bioinformatics analyses including Gene Ontology and KEGG pathway enrichment analysis were performed to evaluate the potential features and capabilities of the identified differentially expressed proteins. Results A total of 51 commonly differentially expressed proteins were identified among the comparisons between mild, moderate, and severe HIE as well as healthy controls. Haptoglobin (HP) and S100A8 were most significantly up-regulated in patients with HIE and further validated via real-time PCR and western blotting. The differentially expressed proteins represented multiple biological processes, cellular components and molecular functions and were markedly enriched in complement and coagulation cascades. Conclusions HP and S100A8 may serve as a potential biomarker for neonatal HIE and reflects the severity of HIE. The complement and coagulation cascades play crucial roles in the development of neonatal HIE. 
546 |a EN 
690 |a Neonate 
690 |a Hypoxic-ischemic encephalopathy 
690 |a Isobaric tags for absolute and relative quantification 
690 |a Haptoglobin 
690 |a S100A8 
690 |a Pediatrics 
690 |a RJ1-570 
655 7 |a article  |2 local 
786 0 |n Italian Journal of Pediatrics, Vol 46, Iss 1, Pp 1-9 (2020) 
787 0 |n http://link.springer.com/article/10.1186/s13052-020-00822-7 
787 0 |n https://doaj.org/toc/1824-7288 
856 4 1 |u https://doaj.org/article/a43a87c69c1e4d3b8f3b728d0338a104  |z Connect to this object online.