Sensitization of TRPV1 by EP<sub>1 </sub>and IP reveals peripheral nociceptive mechanism of prostaglandins
<p>Abstract</p> <p>Prostaglandin E<sub>2 </sub>(PGE<sub>2</sub>) and prostaglandin I<sub>2 </sub>(PGI<sub>2</sub>) are major inflammatory mediators that play important roles in pain sensation and hyperalgesia. The role of their recept...
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| Autores principales: | , , , , , , , , |
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| Formato: | Libro |
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SAGE Publishing,
2005-01-01T00:00:00Z.
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| Acceso en línea: | Connect to this object online. |
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| Sumario: | <p>Abstract</p> <p>Prostaglandin E<sub>2 </sub>(PGE<sub>2</sub>) and prostaglandin I<sub>2 </sub>(PGI<sub>2</sub>) are major inflammatory mediators that play important roles in pain sensation and hyperalgesia. The role of their receptors (EP and IP, respectively) in inflammation has been well documented, although the EP receptor subtypes involved in this process and the underlying cellular mechanisms remain to be elucidated. The capsaicin receptor TRPV1 is a nonselective cation channel expressed in sensory neurons and activated by various noxious stimuli. TRPV1 has been reported to be critical for inflammatory pain mediated through PKA- and PKC-dependent pathways. PGE<sub>2 </sub>or PGI<sub>2</sub>increased or sensitized TRPV1 responses through EP<sub>1 </sub>or IP receptors, respectively predominantly in a PKC-dependent manner in both HEK293 cells expressing TRPV1 and mouse DRG neurons. In the presence of PGE<sub>2 </sub>or PGI<sub>2</sub>, the temperature threshold for TRPV1 activation was reduced below 35°C, so that temperatures near body temperature are sufficient to activate TRPV1. A PKA-dependent pathway was also involved in the potentiation of TRPV1 through EP<sub>4 </sub>and IP receptors upon exposure to PGE<sub>2 </sub>and PGI<sub>2</sub>, respectively. Both PGE<sub>2</sub>-induced thermal hyperalgesia and inflammatory nociceptive responses were diminished in TRPV1-deficient mice and EP<sub>1</sub>-deficient mice. IP receptor involvement was also demonstrated using TRPV1-deficient mice and IP-deficient mice. Thus, the potentiation or sensitization of TRPV1 activity through EP<sub>1 </sub>or IP activation might be one important mechanism underlying the peripheral nociceptive actions of PGE<sub>2 </sub>or PGI<sub>2</sub>.</p> |
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| Notas: | 10.1186/1744-8069-1-3 1744-8069 |