Extended Spectrum Beta-Lactamase-Resistant Determinants among Carbapenem-Resistant <i>Enterobacteriaceae</i> from Beef Cattle in the North West Province, South Africa: A Critical Assessment of Their Possible Public Health Implications
Carbapenems are considered to be the last resort antibiotics for the treatment of infections caused by extended-spectrum beta-lactamase (ESBL)-producing strains. The purpose of this study was to assess antimicrobial resistance profile of Carbapenem-resistant <i>Enterobacteriaceae</i> (CR...
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| Main Authors: | , , , , |
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| Format: | Book |
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MDPI AG,
2020-11-01T00:00:00Z.
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| Summary: | Carbapenems are considered to be the last resort antibiotics for the treatment of infections caused by extended-spectrum beta-lactamase (ESBL)-producing strains. The purpose of this study was to assess antimicrobial resistance profile of Carbapenem-resistant <i>Enterobacteriaceae</i> (CRE) isolated from cattle faeces and determine the presence of carbapenemase and ESBL encoding genes. A total of 233 faecal samples were collected from cattle and analysed for the presence of CRE. The CRE isolates revealed resistance phenotypes against imipenem (42%), ertapenem (35%), doripenem (30%), meropenem (28%), cefotaxime, (59.6%) aztreonam (54.3%) and cefuroxime (47.7%). Multidrug resistance phenotypes ranged from 1.4 to 27% while multi antibiotic resistance (MAR) index value ranged from 0.23 to 0.69, with an average of 0.40. <i>Escherichia coli</i> (<i>E. coli</i>), <i>Klebsiella pneumoniae</i> (<i>K. pneumoniae</i>), <i>Proteus mirabilis</i> (<i>P. mirabilis</i>) and <i>Salmonella</i> (34.4, 43.7, 1.3 and 4.6%, respectively) were the most frequented detected species through genus specific PCR analysis. Detection of genes encoding carbapenemase ranged from 3.3% to 35% (<i>blaKPC</i>, <i>blaNDM</i>, <i>blaGES</i>, <i>blaOXA-48</i>, <i>blaVIM</i> and <i>blaOXA-23</i>). Furthermore, CRE isolates harboured ESBL genes (<i>blaSHV</i> (33.1%), <i>blaTEM</i> (22.5%), <i>blaCTX-M</i> (20.5%) and <i>blaOXA</i> (11.3%)). In conclusion, these findings indicate that cattle harbour CRE carrying ESBL determinants and thus, proper hygiene measures must be enforced to mitigate the spread of CRE strains to food products. |
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| Item Description: | 10.3390/antibiotics9110820 2079-6382 |