The Role of Pigments and Cryptochrome 1 in the Adaptation of <i>Solanum lycopersicum</i> Photosynthetic Apparatus to High-Intensity Blue Light

• Main Authors: Aleksandr Ashikhmin (Author), Pavel Pashkovskiy (Author), Anatoliy Kosobryukhov (Author), Alexandra Khudyakova (Author), Anna Abramova (Author), Mikhail Vereshchagin (Author), Maksim Bolshakov (Author), Vladimir Kreslavski (Author)
• Format: Book
• Published: MDPI AG, 2024-05-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

The effects of high-intensity blue light (HIBL, 500/1000 µmol m⁻²s⁻¹, 450 nm) on <i>Solanum lycopersicum</i> mutants with high pigment (<i>hp</i>) and low pigment (<i>lp)</i> levels and cryptochrome 1 (cry1) deficiency on photosynthesis, chlorophylls, phenols, anthocyanins, nonenzymatic antioxidant activity, carotenoid composition, and the expression of light-dependent genes were investigated. The plants, grown under white light for 42 days, were exposed to HIBL for 72 h. The <i>hp</i> mutant quickly adapted to 500 µmol m⁻²s⁻¹ HIBL, exhibiting enhanced photosynthesis, increased anthocyanin and carotenoids (beta-carotene, zeaxanthin), and increased expression of key genes involved in pigment biosynthesis (<i>PSY1</i>, <i>PAL1</i>, <i>CHS</i>, <i>ANS</i>) and PSII proteins along with an increase in nonenzymatic antioxidant activity. At 1000 µmol m⁻²s⁻¹ HIBL, the <i>lp</i> mutant showed the highest photosynthetic activity, enhanced expression of genes associated with PSII external proteins (<i>psbO</i>, <i>psbP</i>, <i>psbQ</i>), and increased in neoxanthin content. This mutant demonstrated greater resistance at the higher HIBL, demonstrating increased stomatal conductance and photosynthesis rate. The <i>cry1</i> mutant exhibited the highest non-photochemical quenching (NPQ) but had the lowest pigment contents and decreased photosynthetic rate and PSII activity, highlighting the critical role of CRY1 in adaptation to HIBL. The <i>hp</i> and <i>lp</i> mutants use distinct adaptation strategies, which are significantly hindered by the <i>cry1</i> mutation. The pigment content appears to be crucial for adaptation at moderate HIBL doses, while CRY1 content and stomatal activity become more critical at higher doses.

Item Description

10.3390/antiox13050605
2076-3921