Purity of maltose-binding protein - Recombinant streptavidin expressed in Escherichia coli BL21 (pD861-MBP: 327892)

• Main Authors: Toto Subroto (Author), Iman Permana Maksum (Author), Muhammad Yusuf (Author), Sri Agung Fitri Kusuma (Author), Wulan Opratami (Author), Maulida Maharani (Author)
• Formato: Livro
• Publicado em: Wolters Kluwer Medknow Publications, 2022-01-01T00:00:00Z.
• Assuntos: article
• Acesso em linha: Connect to this object online.

Nearly 95% of streptavidin which is expressed in Escherichia coli found as an inclusion body. Protein expressed in an inclusion body form requires further steps for the folding process related to its purification. Whereas the purity level of the recombinant streptavidin is very crucial mainly for th...