Analytical sensitivity and specificity of a loop-mediated isothermal amplification (LAMP) kit prototype for detection of Trypanosoma cruzi DNA in human blood samples.

• Main Authors: Susana A Besuschio (Author), Mónica Llano Murcia (Author), Alejandro F Benatar (Author), Severine Monnerat (Author), Israel Cruz (Author), Albert Picado (Author), María de Los Ángeles Curto (Author), Yutaka Kubota (Author), Diana P Wehrendt (Author), Paula Pavia (Author), Yasuyoshi Mori (Author), Concepción Puerta (Author), Joseph M Ndung'u (Author), Alejandro G Schijman (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2017-07-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

This study aimed to assess analytical parameters of a prototype LAMP kit that was designed for detection of Trypanosoma cruzi DNA in human blood. The prototype is based on the amplification of the highly repetitive satellite sequence of T.cruzi in microtubes containing dried reagents on the inside o...