Antimicrobial Resistance Genes in Porcine <i>Pasteurella multocida</i> Are Not Associated with Its Antimicrobial Susceptibility Pattern
Forty-eight <i>Pasteurella multocida</i> isolates were recovered from porcine pneumonic lungs collected from farms in "Castilla y León" (north-western Spain) in 2017-2019. These isolates were characterized for their minimal inhibition concentrations to twelve antimicrobial agen...
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| Main Authors: | , , , , , |
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| Format: | Book |
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MDPI AG,
2020-09-01T00:00:00Z.
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| Summary: | Forty-eight <i>Pasteurella multocida</i> isolates were recovered from porcine pneumonic lungs collected from farms in "Castilla y León" (north-western Spain) in 2017-2019. These isolates were characterized for their minimal inhibition concentrations to twelve antimicrobial agents and for the appearance of eight resistance genes: <i>tetA</i>, <i>tetB</i>, <i>bla<sub>ROB1</sub></i>, <i>bla<sub>TEM</sub></i>, <i>ermA</i>, <i>ermC</i>, <i>mphE</i> and <i>msrE</i>. Relevant resistance percentages were shown against tetracyclines (52.1% for doxycycline, 68.7% for oxytetracycline), sulphamethoxazole/trimethoprim (43.7%) and tiamulin (25.0%), thus suggesting that <i>P. multocida</i> isolates were mostly susceptible to amoxicillin, ceftiofur, enrofloxacin, florfenicol, marbofloxacin and macrolides. Overall, 29.2% of isolates were resistant to more than two antimicrobials. The tetracycline resistance genes (<i>tetA</i> and <i>tetB</i>) were detected in 22.9% of the isolates, but none were positive to both simultaneously; <i>bla<sub>ROB1</sub></i> and <i>bla<sub>TEM</sub></i> genes were found in one third of isolates but both genes were detected simultaneously in only one isolate. The <i>ermC</i> gene was observed in 41.7% of isolates, a percentage that decreased to 22.9% for <i>msrE</i>; finally, <i>ermA</i> was harbored by 16.7% and <i>mph</i>E was not found in any of them. Six clusters were established based on hierarchical clustering analysis on antimicrobial susceptibility for the twelve antimicrobials. Generally, it was unable to foresee the antimicrobial susceptibility pattern for each family and the association of each particular isolate inside the clusters established from the presence or absence of the resistance genes analyzed. |
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| Item Description: | 10.3390/antibiotics9090614 2079-6382 |