In vitro and in vivo antioxidant potentials of Alchornea floribunda leaf extract, fractions and isolated bioactive compounds

• Main Authors: Daniel Lotanna Ajaghaku (Author), Okechukwu Obasi (Author), Blessing Ogechukwu Umeokoli (Author), Peter Ogbuatu (Author), Chukwuemeka Sylvester Nworu (Author), Emmanuel Emeka Ilodigwe (Author), Festus Basden Chiedu Okoye (Author)
• Format: Book
• Published: Mashhad University of Medical Sciences, 2017-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

Objective: Alchornea floribunda leaves are widely used in ethnomedicine for the management of immuno-inflammatory disorders. We investigated the in vivo and in vitro antioxidant activity of the leaf extract, fractions and isolated compounds of A. floribunda.Materials and Methods: The ethyl acetate fraction of the methanol leaf extract was subjected to several chromatographic separations to isolate compounds 1-4. The structures of the isolated compounds were elucidated by a combination of 1D and 2D NMR and mass spectrometry. Oxidative stress was induced with carbon tetrachloride (CCl4). Further analysis on the isolated phenolic compounds were done using 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP) and hydrogen peroxide scavenging activity tests.Results: The ethyl acetate fraction at 200 mg/kg produced significant (p

Item Description

2228-7930
2228-7949
10.22038/ajp.2016.7003