Method development for quantification of DMET-proteins in the paediatric intestinal tract via LC-MS/MS using a QconCAT technique
Physiologically based pharmacokinetic (PBPK) modelling is a powerful alternative for paediatric clinical trials. Paediatric PBPK models require data on intestinal drug metabolising enzymes and transporter (DMET)-protein abundances, however only limited data is available. This is the first study to r...
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| Main Authors: | , , |
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| Format: | Book |
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University of Huddersfield Press,
2022-11-01T00:00:00Z.
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| Summary: | Physiologically based pharmacokinetic (PBPK) modelling is a powerful alternative for paediatric clinical trials. Paediatric PBPK models require data on intestinal drug metabolising enzymes and transporter (DMET)-protein abundances, however only limited data is available. This is the first study to report paediatric duodenal DMET protein quantification using a QconCAT approach. Thirty-six paediatric intestinal biopsies have been obtained from which the total mucosal protein was extracted. Proteins were digested to peptides using FASP and peptide levels were quantified using LC-MS/MS. Preliminary results provide some insight on the effect of age on duodenal protein abundance. |
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| Item Description: | 10.5920/bjpharm.1166 2058-8356 |