Method development for quantification of DMET-proteins in the paediatric intestinal tract via LC-MS/MS using a QconCAT technique
Physiologically based pharmacokinetic (PBPK) modelling is a powerful alternative for paediatric clinical trials. Paediatric PBPK models require data on intestinal drug metabolising enzymes and transporter (DMET)-protein abundances, however only limited data is available. This is the first study to r...
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| Format: | Book |
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University of Huddersfield Press,
2022-11-01T00:00:00Z.
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| LEADER | 00000 am a22000003u 4500 | ||
|---|---|---|---|
| 001 | doaj_aa7152bf72cf4ee7891aadaa99a27b9c | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a Jan Goelen |e author |
| 700 | 1 | 0 | |a Richard D Horniblow |e author |
| 700 | 1 | 0 | |a Hannah Batchelor |e author |
| 245 | 0 | 0 | |a Method development for quantification of DMET-proteins in the paediatric intestinal tract via LC-MS/MS using a QconCAT technique |
| 260 | |b University of Huddersfield Press, |c 2022-11-01T00:00:00Z. | ||
| 500 | |a 10.5920/bjpharm.1166 | ||
| 500 | |a 2058-8356 | ||
| 520 | |a Physiologically based pharmacokinetic (PBPK) modelling is a powerful alternative for paediatric clinical trials. Paediatric PBPK models require data on intestinal drug metabolising enzymes and transporter (DMET)-protein abundances, however only limited data is available. This is the first study to report paediatric duodenal DMET protein quantification using a QconCAT approach. Thirty-six paediatric intestinal biopsies have been obtained from which the total mucosal protein was extracted. Proteins were digested to peptides using FASP and peptide levels were quantified using LC-MS/MS. Preliminary results provide some insight on the effect of age on duodenal protein abundance. | ||
| 546 | |a EN | ||
| 690 | |a paediatrics | ||
| 690 | |a proteomics | ||
| 690 | |a pbpk modelling | ||
| 690 | |a biopharmaceutics | ||
| 690 | |a Pharmacy and materia medica | ||
| 690 | |a RS1-441 | ||
| 655 | 7 | |a article |2 local | |
| 786 | 0 | |n British Journal of Pharmacy, Vol 7, Iss 2 (2022) | |
| 787 | 0 | |n https://www.bjpharm.org.uk/article/id/1166/ | |
| 787 | 0 | |n https://doaj.org/toc/2058-8356 | |
| 856 | 4 | 1 | |u https://doaj.org/article/aa7152bf72cf4ee7891aadaa99a27b9c |z Connect to this object online. |