Anti-Metastatic Effects on Melanoma via Intravenous Administration of Anti-NF-κB siRNA Complexed with Functional Peptide-Modified Nano-Micelles
Controlling metastasis is an important strategy in cancer treatment. Nanotechnology and nucleic acids with novel modalities are promising regulators of cancer metastasis. We aimed to develop a small interfering RNA (siRNA) systemic delivery and anti-metastasis system using nanotechnology. We previou...
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MDPI AG,
2020-01-01T00:00:00Z.
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| LEADER | 00000 am a22000003u 4500 | ||
|---|---|---|---|
| 001 | doaj_aae82fe17a0849f5b4b67eddb25b1ce6 | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a Hisako Ibaraki |e author |
| 700 | 1 | 0 | |a Takanori Kanazawa |e author |
| 700 | 1 | 0 | |a Minami Owada |e author |
| 700 | 1 | 0 | |a Keiko Iwaya |e author |
| 700 | 1 | 0 | |a Yuuki Takashima |e author |
| 700 | 1 | 0 | |a Yasuo Seta |e author |
| 245 | 0 | 0 | |a Anti-Metastatic Effects on Melanoma via Intravenous Administration of Anti-NF-κB siRNA Complexed with Functional Peptide-Modified Nano-Micelles |
| 260 | |b MDPI AG, |c 2020-01-01T00:00:00Z. | ||
| 500 | |a 1999-4923 | ||
| 500 | |a 10.3390/pharmaceutics12010064 | ||
| 520 | |a Controlling metastasis is an important strategy in cancer treatment. Nanotechnology and nucleic acids with novel modalities are promising regulators of cancer metastasis. We aimed to develop a small interfering RNA (siRNA) systemic delivery and anti-metastasis system using nanotechnology. We previously reported that polyethylene glycol-polycaprolactone (PEG-PCL) and functional peptide CH2R4H2C nano-micelle (MPEG-PCL-CH2R4H2C) has high siRNA silencing effects, indicated by increased drug accumulation in tumor-bearing mice, and has an anti-tumor effect on solid tumors upon systemic injection. In this study, we aimed to apply our micelles to inhibit metastasis and evaluated the inhibitory effect of anti-RelA siRNA (siRelA), which is a subunit of NF-κB conjugated with MPEG-PCL-CH2R4H2C, via systemic administration. We report that siRelA/MPEG-PCL-CH2R4H2C had a high cellular uptake and suppressed the migration/invasion of cells in B16F10 cells without toxicity. In addition, in a lung metastasis mouse model using intravenous administration of B16F10 cells treated with siRelA/MPEG-PCL-CH2R4H2C, the number of lung nodules in lung tissue significantly decreased compared to naked siRelA and siControl/MPEG-PCL-CH2R4H2C micelle treatments. Hence, we show that RelA expression can reduce cancer metastasis, and MPEG-PCL-CH2R4H2C is an effective siRNA carrier for anti-metastasis cancer therapies. | ||
| 546 | |a EN | ||
| 690 | |a polymer nano-micelles | ||
| 690 | |a sirna | ||
| 690 | |a systemic delivery | ||
| 690 | |a metastasis | ||
| 690 | |a melanoma | ||
| 690 | |a nf-κb | ||
| 690 | |a rela | ||
| 690 | |a functional peptide | ||
| 690 | |a Pharmacy and materia medica | ||
| 690 | |a RS1-441 | ||
| 655 | 7 | |a article |2 local | |
| 786 | 0 | |n Pharmaceutics, Vol 12, Iss 1, p 64 (2020) | |
| 787 | 0 | |n https://www.mdpi.com/1999-4923/12/1/64 | |
| 787 | 0 | |n https://doaj.org/toc/1999-4923 | |
| 856 | 4 | 1 | |u https://doaj.org/article/aae82fe17a0849f5b4b67eddb25b1ce6 |z Connect to this object online. |