Identification of estrogen-regulated genes by microarray analysis of the uterus of immature rats exposed to endocrine disrupting chemicals

<p>Abstract</p> <p>Environmental estrogenic compounds which bind to the estrogen receptor (ER) can block or alter endogenous functions of estrogen in reproductive and developmental stages. A microarray technology is a very valuable method for the prediction of hormone-responsive ac...

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Main Authors: Leung Peter CK (Author), Choi Kyung-Chul (Author), Park Se-Hyung (Author), Hong Eui-Ju (Author), Jeung Eui-Bae (Author)
Format: Book
Published: BMC, 2006-09-01T00:00:00Z.
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001 doaj_ab3a8d56ad0e487f8dce1caad0910ef1
042 |a dc 
100 1 0 |a Leung Peter CK  |e author 
700 1 0 |a Choi Kyung-Chul  |e author 
700 1 0 |a Park Se-Hyung  |e author 
700 1 0 |a Hong Eui-Ju  |e author 
700 1 0 |a Jeung Eui-Bae  |e author 
245 0 0 |a Identification of estrogen-regulated genes by microarray analysis of the uterus of immature rats exposed to endocrine disrupting chemicals 
260 |b BMC,   |c 2006-09-01T00:00:00Z. 
500 |a 10.1186/1477-7827-4-49 
500 |a 1477-7827 
520 |a <p>Abstract</p> <p>Environmental estrogenic compounds which bind to the estrogen receptor (ER) can block or alter endogenous functions of estrogen in reproductive and developmental stages. A microarray technology is a very valuable method for the prediction of hormone-responsive activities in various gene expressions. Thus, we investigated the altered gene expression by estrogen and endocrine disruptors (EDs) using microarray technology in the uterus of immature rats. In this study, the expression levels of only 555 genes (7.42%) among the 7636 genes spotted on microarray chips were enhanced by more than two-fold following treatment with estradiol (E2), suggesting that direct or rapid response to E2 is widespread at the mRNA levels in these genes. In addition, elevated expression levels of the genes (over 2-fold) were observed by diethylstilbestrol (DES; 9.01%), octyl-phenol (OP; 8.81%), nonyl-phenol (NP; 9.51%), bisphenol-A (BPA; 8.26%) or genistein (9.97%) in the uterus of immature rats. The expression levels of representative genes, i.e., calbindin-D9k (CaBP-9k; vitamin D-dependent calcium-binding protein), oxytocin, adipocyte complement related protein (MW 30 kDa), lactate dehydrogenase A and calcium binding protein A6 (S100a6; calcyclin), were confirmed in these tissues by real-time PCR. In addition, the mRNA levels of these genes by real-time PCR were increased at follicular phase when E2 level was elevated during estrous cycle of adult female rats. In conclusion, these results indicate distinct altered expression of responsive genes following exposure to E2 and estrogenic compounds, and implicate distinct effects of endogenous E2 and environmental endocrine disrupting chemicals in the uterus of immature rats.</p> 
546 |a EN 
690 |a Gynecology and obstetrics 
690 |a RG1-991 
690 |a Reproduction 
690 |a QH471-489 
655 7 |a article  |2 local 
786 0 |n Reproductive Biology and Endocrinology, Vol 4, Iss 1, p 49 (2006) 
787 0 |n http://www.rbej.com/content/4/1/49 
787 0 |n https://doaj.org/toc/1477-7827 
856 4 1 |u https://doaj.org/article/ab3a8d56ad0e487f8dce1caad0910ef1  |z Connect to this object online.