An Integrated Strategy for Investigating Antioxidants from <i>Ribes himalense</i> Royle ex Decne and Their Potential Target Proteins

• Main Authors: Chuang Liu (Author), Yuqing Lei (Author), Youyi Liu (Author), Jingrou Guo (Author), Xingyi Chen (Author), Yifei Tang (Author), Jun Dang (Author), Minchen Wu (Author)
• Format: Book
• Published: MDPI AG, 2023-03-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

Natural products have been used extensively around the world for many years as therapeutic, prophylactic, and health-promotive agents. <i>Ribes himalense</i> Royle ex Decne, a plant used in traditional Tibetan medicine, has been demonstrated to have significant antioxidant and anti-inflammatory properties. However, the material basis of its medicinal effects has not been sufficiently explored. In this study, we established an integrated strategy by online HPLC-1,1-diphenyl-2-picrylhydrazyl, medium-pressure liquid chromatography, and HPLC to achieve online detection and separation of antioxidants in <i>Ribes himalense</i> extracts. Finally, four antioxidants with quercetin as the parent nucleus were obtained, namely, Quercetin-3-O-β-D-glucopyranoside-7-O-α-L-rhamnopyranoside, Quercetin-3-O-β-D-xylopyranosyl(1-2)-β-D-glucopyranoside, Quercetin-3-O-β-D-glucopyranoside, and Quercetin-3-O-β-D-galactoside. Until now, the four antioxidants in <i>Ribes himalense</i> have not been reported in other literatures. Meanwhile, the free-radical-scavenging ability of them was evaluated by DPPH assay, and potential antioxidant target proteins were explored using molecular docking. In conclusion, this research provides insights into the active compounds in <i>Ribes himalense</i> which will facilitate the advancement of deeper studies on it. Moreover, such an integrated chromatographic strategy could be a strong driver for more efficient and scientific use of other natural products in the food and pharmaceutical industries.

Item Description

10.3390/antiox12040835
2076-3921