Emergence and Genomic Features of a <i>mcr-1 Escherichia coli</i> from Duck in Hungary

• Huvudupphovsmän: Ama Szmolka (Författare, medförfattare), Ákos Gellért (Författare, medförfattare), Dóra Szemerits (Författare, medförfattare), Fanni Rapcsák (Författare, medförfattare), Sándor Spisák (Författare, medförfattare), András Adorján (Författare, medförfattare)
• Materialtyp: Bok
• Publicerad: MDPI AG, 2023-10-01T00:00:00Z.
• Ämnen: article
• Länkar: Connect to this object online.
• Notes: 10.3390/antibiotics12101519; 2079-6382
• Keywords: <i>mcr-1 Escherichia coli</i>; colistin resistance; waterfowl; plasmid genome; core genome MLST; multiresistance; Therapeutics. Pharmacology; RM1-950
• Source: Antibiotics, Vol 12, Iss 10, p 1519 (2023)

Plasmids carrying high-risk resistance mechanisms in pathogenic <i>E. coli</i> have gained particular attention in veterinary medicine, especially since the discovery of the colistin resistance gene, <i>mcr-1</i>. Here, we provide the first evidence of its emergence and describe the complete <i>mcr-1</i> plasmid sequence of a multi-resistant avian pathogenic <i>E. coli</i> (APEC) strain from waterfowl in Hungary. Whole-genome sequencing analysis and core-genome MLST were performed to characterize the genome structure of the <i>mcr-1</i> plasmid and to reveal the phylogenetic relation between the Hungarian duck strain Ec45-2020 and the internationally circulating <i>mcr-1</i>-positive <i>E. coli</i> strains from poultry and humans. Results showed that plasmid pEc45-2020-33kb displayed a high level of genome identity with <i>mcr-1</i> plasmids of IncX4 type widespread among human, animal and food reservoirs of enteric bacteria of public health. The <i>mcr-1</i>-positive <i>E. coli</i> strain Ec45-2020 belongs to the ST162 genotype, considered as one of the globally disseminated zoonotic genotypes of MDR <i>E. coli</i>. In accordance with international findings, our results underline the importance of continuous surveillance of enteric bacteria with high-risk antimicrobial resistance genotypes, including neglected animals, such as waterfowls, as possible reservoirs for the colistin resistance gene <i>mcr-1</i>.