From Shadows to Spotlight: Enhancing Bacterial DNA Detection in Blood Samples through Cutting-Edge Molecular Pre-Amplification

• Main Authors: Martin Reinicke (Author), Sascha Daniel Braun (Author), Celia Diezel (Author), Oliver Lemuth (Author), Ines Engelmann (Author), Theresa Liebe (Author), Ralf Ehricht (Author)
• Format: Book
• Published: MDPI AG, 2024-02-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

One of the greatest challenges to the use of molecular methods for diagnostic purposes is the detection of target DNA that is present only in low concentrations. One major factor that negatively impacts accuracy, diagnostic sensitivity, and specificity is the sample matrix, which hinders the attainm...