New ligands of the Cellular Retinoic Acid-Binding Protein 2 (CRABP2) suggest a role for this protein in chromatin remodeling

• Main Authors: Daniella Barros Rossetto (Author), Eric Nishimura Kiyomoto (Author), Carlos Eduardo Brantis de Carvalho (Author), Cleslei Fernando Zanelli (Author), Sandro Roberto Valentini (Author)
• Format: Book
• Published: São Paulo State University (UNESP), 2014-07-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 1808-4532; 2179-443X
• Keywords: crabp2. saccharomyces cerevisiae and twohybrid system.; Pharmaceutical industry; HD9665-9675; Pharmacy and materia medica; RS1-441
• Source: Revista de Ciências Farmacêuticas Básica e Aplicada, Vol 35, Iss 3 (2014)

Retinoic acid (RA) regulates the transcription of a series of genes involved in cell proliferation, differentiation and apoptosis by binding to the RA Receptor (RAR) and Retinoid X Receptor (RXR) heterodimers. The cellular retinoic acid-binding protein 2 (CRABP2) is involved in the transport of RA from the cytosol to specific RA receptors in the nucleus, acting as a coactivator of nuclear retinoid receptors. In order to have a better understanding of the role of CRABP2 in RA signaling, we used the yeast two-hybrid system as a tool for the identification of physical protein-protein interactions. Twenty-three putative CRABP2-interacting proteins were identified by screening in the presence of RA, five of which are related to transcription regulation or, more specifically, to the process of chromatin remodeling: t-complex 1 (TCP1); H3 histone, family 3A (H3F3A); H3 histone, family 3B (H3F3B); β-tubulin (TUBB) and SR-related CTD-associated factor 1 (SCAF1). These results suggest a more direct role for CRABP2 in chromatin remodeling and may be a starting point for the elucidation of the fine-tuning control of transcription by RA receptors.