Application of PCR-SSP method for HLA-B*27 identification as an auxiliary tool for diagnosis of ankylosing spondylitis

• Main Authors: Gisele Cristina A. Oliveira (Author), Eliane P. Ambrosio-Albuquerque (Author), Jeane Eliete L. Visentainer (Author)
• Format: Book
• Published: Sociedade Brasileira de Patologia Clínica.
• Subjects: article
• Online Access: Connect to this object online.

Summary

ABSTRACT INTRODUCTION: Human leukocyte antigens (HLA) are molecules that present antigen to the immune system; their presence or absence have been described as an influential factor in some diseases. HLA-B*27 is an HLA polymorphism that has been associated with increased susceptibility to ankylosing spondylitis (AS) and other spondyloarthropaty. The detection of HLA-B*27 has been used as diagnostic and prognostic tool in these cases, as well as in the differential diagnosis of other diseases. OBJECTIVE: Standardize the single specific primer-polymerase chain reaction (PCR-SSP) methodology for use in the Immunogenetics Laboratory of the Universidade Estadual de Maringá (UEM), considering its specificity and cost-effectiveness. MATERIAL AND METHODS: A total of 30 individuals without AS positive for HLA-B*27 allele and 10 AS negative individuals, were previously tested by PCR-sequence specific oligonucleotide (PCR-SSO) and, in this study, by PCR-SSP. RESULTS: One hundred percent of the patients tested confirmed their results, even with different subtypes. CONCLUSION: Considering the high reproducibility and the broad spectrum of subtypes covering, it was concluded that the PCR-SSP identifying method for HLA-B*27 can be used as a routine diagnostic tool for spondyloarthropathies.

Item Description

1676-2444
1678-4774
10.5935/1676-2444.20160036